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Purified anti-mouse IFN-β Antibody

Pricing & Availability
Clone
Poly5192 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-β, Fibroblast interferon (Fi-IFN, F-IFN), Type-1 interferon, acid-stable interferon, R1-G1 factor, IFN-b, IFN-beta
Isotype
Goat Polyclonal Ig
Ave. Rating
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Product Citations
publications
IFNb_Mouse_Antibody_ELISA_012814
  • IFNb_Mouse_Antibody_ELISA_012814
Cat # Size Price Quantity Check Availability Save
519202 100 µg $350
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Description

Interferon-β is a member of the type I interferon class. Type I IFNs are involved in the innate immune system, being released non-specifically during the early stage of infection in response to stimuli such as viruses, dsRNA, LPS, and bacterial components. Once secreted by virus-infected or activated cells, they bind to the IFNα/β receptor (IFNAR), which is found on almost all cell types, and, via the JAK-STAT pathway, induce the expression of hundreds of IFN-stimulated genes (ISGs). While the function of many of these proteins remains to be elucidated, a hallmark of type I IFNs is their anti-viral activities, both general and specific against particular viruses. Type I IFNs also regulate cell growth and apoptosis, and exert various immunomodulatory and inflammatory effects. As such, they are also clinically relevant, both as therapeutics and as targets of inhibition for the treatment of various autoimmune diseases and cancer.

Mouse IFN-β is a ~20 kD monomeric glycoprotein. It has 50% amino acid sequence homology with mouse IFN-α, its closest related protein, and 62% homology with human IFN-β. IFN-β is currently the therapeutic of choice for treating multiple sclerosis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Mouse IFN-β
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. A concentration of 0.5 µg/ml of the capture antibody was utilized to generate the example standard curve. It is recommended that each lot of reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: To measure IFN-β, the purified Poly5192 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated MIB-5E9.1 antibody (Cat. No. 508105) as the detection antibody and Recombinant Mouse IFN-β (ELISA Std.) (Cat. No. 581309) as the standard.

Note: For testing mouse IFN-β in serum, plasma or cell culture supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. No. 439407 & 439408) are specially developed and recommended.

Product Citations
  1. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
  2. Cheng Y, et al. 2018. J Exp Med. 215:2919. PubMed
  3. Wu J, et al. 2020. Immunity. 53:115. PubMed
  4. Kalkar P, et al. 2022. Front Pharmacol. 13:1002550. PubMed
  5. Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed
  6. Clua P, et al. 2017. Front Immunol. 10.3389/fimmu.2017.00948. PubMed
  7. Kirk SG, et al. 2021. J Immunol. 206:2966. PubMed
RRID
AB_2561348 (BioLegend Cat. No. 519202)

Antigen Details

Structure
Interferon; 26-35 kD (Mammalian)
Distribution

Fibroblasts, epithelial cells

Interaction
Broad, includes NK cells, T suppressor cells, activated monocytes
Ligand/Receptor
IFNα/βR (IFNAR-1, IFNAR-2)
Cell Type
Epithelial cells, Fibroblasts
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nagarajan U. 2011. Crit. Rev. Immunol. 31:459.
2. Honda K, et al. 2005. Int. Immunol. 17:1367.

Regulation
Upregulated by viruses, double-stranded RNA, micro-organisms, TNF, IL-1
Gene ID
15977 View all products for this Gene ID
UniProt
View information about IFN-beta on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/15/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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  • Purified anti-mouse IFN-β

    IFNb_Mouse_Antibody_ELISA_012814
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