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Purified anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
AN-18 (See other available formats)
Regulatory Status
RUO
Other Names
IFN-gamma
Isotype
Rat IgG1, κ
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Product Citations
publications
AN-18_040711
  • AN-18_040711
Cat # Size Price Quantity Check Availability Save
517901 50 µg $89
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517902 500 µg $250
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse IFN-γ protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
ELISPOT, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5 - 4.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein (Cat. No. 575309) are recomended at concentration up to 2000 pg/mL. The final standard range should be determined by the end-user.. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified AN-18 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as detection antibody, and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture.

Additional reported applications (for the relevant formats) include: immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Prat M, et al. 1984. P. Natl. Acad. Sci. USA 81:4515. (IP)
  2. Kort JJ, et al. 2006. J. Neuroimmunol. 173:23. (ELISPOT) PubMed
  3. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
Product Citations
  1. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  2. Qin J, et al. 2023. Front Immunol. 14:1126392. PubMed
  3. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  4. Adhikari AS, et al. 2022. Front Oncol. 12:850546. PubMed
  5. Ranjan K, et al. 2021. J Clin Invest. 131:. PubMed
  6. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
RRID
AB_10900809 (BioLegend Cat. No. 517901)
AB_10900809 (BioLegend Cat. No. 517902)

Antigen Details

Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Other Formats

View All IFN-γ Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse IFN-γ AN-18 ELISA Capture,ELISPOT,IP
Ultra-LEAF™ Purified anti-mouse IFN-γ AN-18 ELISA Capture,ELISPOT Capture,IP
Go To Top Version: 3    Revision Date: 04/23/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse IFN-γ

    AN-18_040711

  • Ultra-LEAF™ Purified anti-mouse IFN-γ

    AN-18_040711.jpg
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