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Purified anti-mouse IL-4 (Maxpar® Ready) Antibody

Pricing & Availability
Clone
11B11 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-4, Ia inducing factor (IaIF), B cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
11B11_Purified_IL-4_CyTOF_1_112113
C57BL/6 mouse splenocytes were incubated for 20 hours in media alone (top) or with LPS (bottom) in the presence of monensin and brefeldin A. Cells were then fixed, permeabilized, and stained with 166Er-anti-IL4 (11B11). Monocytes are displayed in the analysis. Data provided by DVS Sciences.
  • 11B11_Purified_IL-4_CyTOF_1_112113
    C57BL/6 mouse splenocytes were incubated for 20 hours in media alone (top) or with LPS (bottom) in the presence of monensin and brefeldin A. Cells were then fixed, permeabilized, and stained with 166Er-anti-IL4 (11B11). Monocytes are displayed in the analysis. Data provided by DVS Sciences.
  • 11B11_Purified_IL-4_CyTOF_2_112113
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504129 100 µg $141
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Description

IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partially purified native mouse IL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

ELISA1,2,10,13 or ELISPOT5 Capture: The purified 11B11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD6-24G2 antibody (Cat. No. 504202) as the detecting antibody and recombinant mouse IL-4 (Cat. No. 575609) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization1-2,9,12: The 11B11 antibody can neutralize the bioactivity of natural or recombinant IL-4. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo and in vitro (Cat. No. 504108).
Additional reported applications (for the relevant formats) include: immunoprecipitation16, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections6,7, and immunocytochemistry4.
Note: For testing mouse IL-4 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431101 to 431106) are specially developed and recommended.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Shirai A, et al. 1994. Cytokine 6:329. (ELISA, Neut)
  2. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA, Neut)
  3. Assenmacher M, et al. 1994. Eur. J. Immunol. 24:1097.
  4. Openshaw P, et al. 1995. J. Exp. Med. 182:1357. (ICC)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISA Capture)
  6. Litton M, et al. 1994. J. Immunol. Methods 175:47. (IHC)
  7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
  8. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  9. Hara M, et al. 2001. J. Immunol. 166:3789. (Neut)
  10. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
  11. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  12. Wang W, et al. 2007. J. Immunol. 178:4885. (Neut)
  13. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
  14. Ohnmacht C, et al. 2008. Blood 113:2816. PubMed
  15. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  16. Zavorotinskaya T, et al. 2003. Mol. Ther. 7:155. (IP)
Product Citations
  1. McDonald B, et al. 2020. Cell Host Microbe. 28(5):660-668.e4. PubMed
RRID
AB_2562843 (BioLegend Cat. No. 504129)

Antigen Details

Structure
Cytokine; 15-19 kD (Mammalian)
Bioactivity
Differentiation of naïve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources
Mast cells, T cells, bone marrow stromal cells
Cell Targets
B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors
Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.

Regulation
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID
16189 View all products for this Gene ID
UniProt
View information about IL-4 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Go To Top Version: 3    Revision Date: 07/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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