Purified anti-mouse P2X7R Antibody anti-P2X7R

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Clone: 1F11 (See other available formats)
Regulatory Status: RUO
Other Names: P2X7 receptor (P2X7R), P2RX7
Isotype: Rat IgG2b, κ
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Product Citations: publications

C57BL/6 splenocytes were stained with CD4 FITC and purified P2X7 receptor (clone 1F11, top) or rat IgG2b, κ isotype control (bottom) followed by biotinylated anti-rat IgG2b and SAv-PE.

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148702

100 µg

$311

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Description

P2X7R, also known as P2X7 receptor, belongs to the family of ligand-gated ion channel receptors. It is expressed on T cells, B cells, macrophages, and microglia. The receptor opens in the presence of extracellular ATP or NAD, leading to intracellular calcium mobilization. P2X7R activation requires higher concentrations of ATP compared to other P2X receptors. Longer stimulation results in larger pores, allowing passage of larger molecules. Activation of these molecules also leads to mitochondrial and cytoskeletal changes as well as IL-1β maturation and release. Ligation of P2X7 receptor can lead to membrane blebbing and cell death.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine colon mast cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
IP, IHC, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications for the relevant formats include: immunoprecipitation¹, Western blotting¹, immunohistochemistry¹, in vivo inhibition of intestinal inflammation and mast cell activation¹.
RRID: AB_2564122 (BioLegend Cat. No. 148702)

Antigen Details

Structure: Ligand-gated ion channel, trimer - two transmembrane regions and one cytosolic region.
Distribution: Immune cells, including mast cells, microglia, macrophages, T cells, and B cells.
Function: Ion channel activated by extracellular ATP.
Ligand/Receptor: ATP, NAD.
Cell Type: B cells, Macrophages, Mast cells, T cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Antigen References: 1. Surprenant A, et al. 1996. Science. 272:735.
2. Chused TM, et al. 1996. J. Immunol. 157:1371.
3. Gargett CE, et al. 1997. Br. J. Pharmacol. 122:911.
4. Kawamura H, et al. 2006. J. Immunol. 176:2152.
5. Pelegrin P. 2011. Br. J. Pharmacol. 163:908.
6. Bartlett R, et al. 2014. Pharmacol. Rev. 66:638.
7. Alves LA, et al. 2014. Biochim. Biophys. Acta. 1838:2578.
Gene ID: 18439 View all products for this Gene ID
UniProt: View information about P2X7R on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All P2X7R Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse P2X7R	1F11	FC,IP,WB,IHC
APC anti-mouse P2X7R	1F11	FC
PE anti-mouse P2X7R	1F11	FC
PE/Cyanine7 anti-mouse P2X7R	1F11	FC
PerCP/Cyanine5.5 anti-mouse P2X7R	1F11	FC
TotalSeq™-A0824 anti-mouse P2X7R	1F11	PG
TotalSeq™-C0824 anti-mouse P2X7R	1F11	PG
TotalSeq™-B0824 anti-mouse P2X7R Antibody	1F11	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 08/13/2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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