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- Clone
- A17207D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PD-1 homolog, Dies1, B7-H5
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse PD-1H (VISTA) (clone A17207D) and anti-mouse CD3ε (clone 500A2) Alexa Fluor® 594 (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 647 anti-mouse IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 161302 | 100 µg | $112 | ||||
PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein, composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse VISTA
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-F - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2876577 (BioLegend Cat. No. 161302)
Antigen Details
- Structure
- Type I transmembrane protein, member of the CD28 family
- Distribution
-
Macrophages, dendritic cells, neutrophils, NK cells, naïve and activated T cells
- Function
- Negative regulator of the immune response
- Interaction
- Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
- Cell Type
- Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
- Biology Area
- Immunology
- Antigen References
-
- Flies DB, et al. 2011. J Immunol. 187:1537-41.
- Wang Li, et al. 2011. J Exp Med. 208:577-92.
- Gene ID
- 74048 View all products for this Gene ID
- UniProt
- View information about PD-1H on UniProt.org
Related FAQs
Other Formats
View All PD-1H Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse PD-1H (VISTA) | A17207D | IHC-F |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse PD-1H (VISTA)
C57BL/6 mouse frozen spleen section was fixed with 4% parafo...
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