Purified anti-mouse Qa-2 Antibody anti-Qa-2

Pricing & Availability

Clone: 695H1-9-9 (See other available formats)
Regulatory Status: RUO
Other Names: Nonclassical MHC-Ib, H2-Qa2
Isotype: Mouse IgG2a, κ
Ave. Rating: Submit a Review
Product Citations: publications

695H1-9-9_PURE_Qa-2_Antibody_FC_012417 — C57BL/6 mouse splenocytes were stained with purified anti-mouse Qa-2 (clone-695H1-9-9, filled histogram) or mouse IgG2a, κ isotype control (open histogram) followed by anti-mouse IgG2a, κ FITC.

Compare all formats

Cat #

Size

Price

Quantity Check Availability

Save

121711

500 µg

$293

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

Mouse Qa-2 is a 40 kD GPI-linked surface protein. It is a member of nonclassical MHC class Ib molecule encoded by Q5, Q6, Q7, Q9, and Q10 genes of the H-2 complex. Qa-2 is a nonpolymorphic molecule primarily expressed on mouse T and B cells. The expression level of Qa-2 on mature T cells is higher than on B cells. Different mouse strains express Qa-2 at varying levels. Qa-2, in association with β2-microglobulin, binds to peptide and plays a role in antigen presentation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C3H.SW mouse skin graft and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation².
RRID: AB_2650759 (BioLegend Cat. No. 121711)

Antigen Details

Structure: GPI-linked protein, associates with β2-microglobulin, 40 kD
Distribution: T and B cells, mature T cells expression higher than B cells
Function: Antigen presentation, promote immune response
Ligand/Receptor: Peptide
Cell Type: B cells, T cells
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: 1. Sharrow SO, et al. 1989. J. Immunol. 142:3495.
2. Mann DW, et al. 1987. J. Immunol. 138:240.
3. Mellor AL, et al.1985. Proc. Natl. Acad. Sci.USA. 82:5920.
4. Tabaczewski P, et al. 1997. J. Immunol. 159:2771.
5. Chiang EY and Stroynowski I. 2005. J. Immunol. 174:5367.
Gene ID: 111458 View all products for this Gene ID
UniProt: View information about Qa-2 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All Qa-2 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse Qa-2	695H1-9-9	FC
Alexa Fluor® 647 anti-mouse Qa-2	695H1-9-9	FC
FITC anti-mouse Qa-2	695H1-9-9	FC
Purified anti-mouse Qa-2	695H1-9-9	FC
APC anti-mouse Qa-2	695H1-9-9	FC
PE anti-mouse Qa-2	695H1-9-9	FC

Certificate of Analysis

Go To Top Version: 0 Revision Date: 01/24/2017

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.