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Purified anti-mouse TCR Vγ4 Antibody

Pricing & Availability
Clone
49.2 (See other available formats)
Regulatory Status
RUO
Other Names
T Cell Receptor Vγ4, TCR Vγ2, TRGV4, GV3S1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
49-2_PURE_TCR-Vy4_Antibody_082420
C57BL/6 splenocytes were stained with purified anti-mouse TCR Vγ4 (clone 49.2) (left) or purified mouse IgG1, κ isotype control (right) followed by rat anti-mouse IgG1 PE. Cells were co-stained with anti-CD3 FITC and anti-γδ TCR APC. Data shown are CD3+ gated cells.
  • 49-2_PURE_TCR-Vy4_Antibody_082420
    C57BL/6 splenocytes were stained with purified anti-mouse TCR Vγ4 (clone 49.2) (left) or purified mouse IgG1, κ isotype control (right) followed by rat anti-mouse IgG1 PE. Cells were co-stained with anti-CD3 FITC and anti-γδ TCR APC. Data shown are CD3+ gated cells.
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160602 500 µg $581
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and β chain (TCR α/β) or a γ and δ chain (TCR γ/δ). TCR associates with CD3 to form a CD3/TCR complex. The CD3/TCR plays a key role in antigen recognition, signal transduction, and T cell activation. γδ T cells (gamma delta T cells) are a small subset of T cells that express a unique TCR. They are found in the thymus, peripheral lymphoid tissue, and peritoneum. Unlike αβ T cells, γδ T cells do not require antigen processing in order to recognize certain bacterial, lipid, and tumor antigens.

The 49.2 antibody reacts specifically with mouse TCR Vγ4 as designated by Heilig/Tonegawa nomenclature.2 Mouse TCR Vγ4 is also known as TCR Vγ2 under the Garman nomenclature system. Several TCR γ and δ chain nomenclature systems exist.

In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts.
 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL/6 thymocytes expanded in vitro in anti-TCRγδ antibody coated plates
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Khairallah C, et al. 2015. PLoS Pathog. 11:e1004702.
  2. Perira P, et al. 2000. Eur J Immunol. 30:1988-97.
RRID
AB_2876572 (BioLegend Cat. No. 160602)

Antigen Details

Structure
Heterodimer with γ and δ chain
Distribution

Subset of γδ T cells

Function
Antigen recognition
Interaction
CD3
Cell Type
Lymphocytes, T cells, Thymocytes
Biology Area
Adaptive Immunity, Immunology, Innate Immunity
Molecular Family
TCRs
Gene ID
21638 View all products for this Gene ID
UniProt
View information about TCR Vgamma4 on UniProt.org
Go To Top Version: 2    Revision Date: 11/06/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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