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Purified anti-mouse VEGFR-3 (FLT-4) Antibody

Pricing & Availability
Clone
AFL4 (See other available formats)
Regulatory Status
RUO
Other Names
Vascular endothelial growth factor receptor 3, FLT-4, Fms-like tyrosine kinase 4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
AFL4_Pure_0_061919
Total cell lysates (15 µg total protein) from HEK293T cells mock-transfected or transfected with a plasmid containing FLT4 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-VEGFR-3 (FLT-4) Antibody, clone AFL4, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • AFL4_Pure_0_061919
    Total cell lysates (15 µg total protein) from HEK293T cells mock-transfected or transfected with a plasmid containing FLT4 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-VEGFR-3 (FLT-4) Antibody, clone AFL4, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • AFL4_Pure_1_111710
    6 µm frozen thymus section from a naïve C57BL/6 mouse was stained with purified anti-VEGFR-3 (clone AFL4), followed by incubation with goat anti-rat IgG Alexa Fluor® 488 (green) and DAPI (blue). Magnification 40X.
  • AFL4_Pure_2_111710
    6 µm frozen lung section from an influenza-infected C57BL/6 mouse stained with purified anti-VEGFR-3 (clone AFL4), followed by incubation with goat anti-rat IgG Alexa Fluor® 488 (green), anti-CD3 (red) and DAPI (blue). Magnification 40X.
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140901 50 µg $83
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140902 500 µg $258
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Description

VEGFR-3, also known as FLT4, is a member of the Tyr protein kinase family. The extracellular portion of VEGFR-3 contains 7 immunoglobulin (Ig)-like domains and the cytoplasmic portion contains a protein kinase domain. FLT4 regulates angiogenesis and lymphangiogenesis, its ligands are VEGF-C and D and its binding is mediated by the 2nd and 3rd Ig-like domains of FLT4. During fetal development VEGFR-3 is expressed on endothelial cells, however, in the adult mice, the vascular endothelial cells lose VEGFR-3 expression, but the lymphatic endothelium expresses it constitutively. In addition, VEGFR-3 expression can be induced in tumors with active angiogenesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
VEGFR-3-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Block, ELISA, IP, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA1,2, immunoprecipitation1and Western blot1,2 from cell lysates, immunohistochemical staining1, 3 of frozen and paraffin embedded tissue sections and in whole-mount mouse embryos, in vivo blocking1, 5 of VEGF-C binding to inhibit angiogenesis, lymphangiogenesis, growth and metastasis of different tumor cells, in vitro blocking4 of VEGF-C binding to induce dispersion of endothelial cell clusters.

This clone detect multiple bands by western blot: a ~140 kD band corresponding to cleaved/soluble VEGFR-3, and 220 and 230 kD bands corresponding to unglycosylated and glycosylated full length VEGFR-3 (Bando, et al. 2004. Int J Canc. 111:184.)

Additional Product Notes

This product has been reported by a collaborator to work for immunohistochemical staining of frozen tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Kubo H, et al. 2000. Blood 96:546. (IP, WB, IHC, ELISA, Block)
  2. Zhang L, et al. 2010. Cell. Res. Epub ahead of print (WB, ELISA)
  3. Tammela T, et al. 2010. Nature 454:656. (IHC)
  4. Shimizu K, et al. 2004. Cancer Sci. 95:328. (Block)
  5. Matsumura K, et al. 2003. Blood 101:1367. (Block)
  6. Lee J, et al. 2012. PNAS 109:19638. PubMed
  7. Ozasa R, et al. 2012. J Exp Clin Cancer Res. 31:83. PubMed
Product Citations
  1. Ozasa R, et al. 2012. J Exp Clin Cancer Res. 31:83. PubMed
  2. Bieniasz–Krzywiec P, et al. 2020. Cell Metabolism. 30(5):917-936. PubMed
  3. Inverso D, et al. 2021. Developmental Cell. 56(11):1677-1693.e10. PubMed
  4. Lee J, et al. 2012. Proc Natl Acad Sci U S A. 109:19638. PubMed
RRID
AB_10680790 (BioLegend Cat. No. 140901)
AB_10680790 (BioLegend Cat. No. 140902)

Antigen Details

Structure
Member of Tyr protein kinase family. Extracellular portion contains 7 immunoglobulin-like domains. Ligand binding is mediated by its domains 2 and 3. Cytoplasmic portion contains 1 protein kinase domain.
Distribution

Expressed on vascular endothelial cells during fetal development, constitutively expressed on lymphatic endothelium in adult mice, induced in tumors with active angiogenesis.

Function
Regulates angiogenesis and lymphangiogenesis.
Interaction
Associated with VEGFR-2 in the surface.
Ligand/Receptor
VEGF-C and D
Cell Type
Endothelial cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors, Protein Kinases/Phosphatase
Antigen References

1. Uhrin P, et al. 2010. Blood 115:3997.
2. Wang Y, et al. 2010. Nature 465:483.
3. Akobsson L, et al. 2009. Biochem. Soc. T. 37:1233.
4. Tammela T, et al. 2010. Nature 454:656.
5. Huang G and Chen L. 2008. Cancer Biother. Radio. 23:661.
6. Zhang YJ and Fang JY. 2008. J. Gastroen. Hepatol. 23:856.

Gene ID
14257 View all products for this Gene ID
UniProt
View information about VEGFR-3 on UniProt.org
Go To Top Version: 3    Revision Date: 06/27/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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