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- Clone
- A16007A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NRGN, Ng, RC3, Calmodulin-Binding Protein, Protein Kinase C Substrate, Hng
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
IHC staining of purified anti-Neurogranin antibody (clone A16007A) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol. -
Western blot of purified anti-Neurogranin antibody (clone A16007A) and isotype-matched IgG2b control, demonstrating antibody specificity towards endogenous neurogranin protein. Lane 1: 20 µg of human brain tissue lysate; Lane 2: 20 µg of human Alzheimer's disease brain tissue lysate; Lanes 3 and 4: 5 µg of rat brain tissue lysate; Lane 5: 50 ng of human recombinant Neurogranin-GST protein. Blots were incubated with 1 µg/mL of the primary antibody overnight at 4°C followed by incubation with HRP labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 848501 | 25 µg | $106 | ||||
| 848502 | 100 µg | $265 | ||||
Neurogranin (NRGN) is the main post-synaptic protein kinase substrate that binds to calmodulin (CaM) in the absence of calcium. It is suggested that NRGN is a direct target for thyroid hormone in the human brain, and that the control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. Neurogranin in CSF has been implicated as a possible marker for synaptic degeneration in Alzheimer’s disease.
Product DetailsProduct Details
- Verified Reactivity
- Human, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH conjugated synthetic peptide corresponding to amino acid residues 70-78 of human neurogranin protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ELISA Capture, IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2650691 (BioLegend Cat. No. 848501)
AB_2650691 (BioLegend Cat. No. 848502)
Antigen Details
- Structure
- Neurogranin is a 78 amino acid protein with a molecular mass of approximately 7.6 kD.
- Distribution
-
Tissue Distribution: Cerebral cortex neurons in layers II-VI, and pyramidal neurons.
Cellular distribution: Golgi apparatus, mitochondria, nucleus, and cytoplasm in neuronal cell body and dendrites. - Function
- Neurogranin is a neuron-specific, calmodulin-binding protein that participates in the protein kinase C signaling pathway. Neurogranin is a post-synaptic protein that modulates Ca2+ homeostasis in neurons by binding to and regulating the availability of calmodulin in the absence of Ca2+. Neurogranin function has been implicated in learning and memory.
- Ligand/Receptor
- Calmodulin.
- Cell Type
- Neurons
- Biology Area
- Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Antigen References
-
1. Diez-Guerra FJ. 2010. IUBMB Life. 62(8): 597. (PubMed)
2. De Vos A, et al. 2016. J Alzheimers Dis. 53(4): 1523. (PubMed)
3. De Vos A. et al. 2015. Alzheimers Dement. 11(12):1461. (PubMed) - Gene ID
- 4900 View all products for this Gene ID
- UniProt
- View information about Neurogranin 70-78 on UniProt.org
Other Formats
View All Neurogranin, 70-78 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Neurogranin, 70-78 | A16007A | WB,ELISA Capture,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Neurogranin, 70-78
IHC staining of purified anti-Neurogranin antibody (clone A1... 
Western blot of purified anti-Neurogranin antibody (clone A1...
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