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Purified anti-NF-κB p105/p50 Antibody

Pricing & Availability
Clone
W16200A (See other available formats)
Regulatory Status
RUO
Other Names
Nuclear factor NF-kappa-B p105/p50 subunit, DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Isotype
Rat IgG2a, κ
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Product Citations
publications
W16200A_PURE_NF-kB_Antibody_092817
Total lysates (15 µg protein) from HeLa, Molt-4 and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti- NF-κB p105/p50 antibody (clone W16200A, upper) or GAPDH (clone Poly6314, Cat. No. 631401) antibody (lower). Proteins were visualized using an HRP goat anti-rat-IgG (clone Poly4054, Cat. No. 405405) secondary antibody for NFKB1 or HRP Donkey anti-rabbit IgG (clone Poly4064, Cat. No. 406401) antibody for GAPDH and chemiluminescence detection. Lane M is the MW ladder.
  • W16200A_PURE_NF-kB_Antibody_092817
    Total lysates (15 µg protein) from HeLa, Molt-4 and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti- NF-κB p105/p50 antibody (clone W16200A, upper) or GAPDH (clone Poly6314, Cat. No. 631401) antibody (lower). Proteins were visualized using an HRP goat anti-rat-IgG (clone Poly4054, Cat. No. 405405) secondary antibody for NFKB1 or HRP Donkey anti-rabbit IgG (clone Poly4064, Cat. No. 406401) antibody for GAPDH and chemiluminescence detection. Lane M is the MW ladder.
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603901 25 µg $101
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603902 100 µg $253
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Description

NF-κB p105/p50 is the class 1 member of the nuclear factor of kappa light polypeptide gene enhancer in B-cells (NF-κB) transcription factor family. NFKB1 is synthesized as a longer precursor protein, p105, which can be prolytically processed into shorter proteins, p50 and IκBγ. Both p105 and IκBγ proteins contain IκB-like ankyrin repeats that can act as NF-κB inhibitors. P50 protein forms active DNA-binding heterodimers with RelA/NF-κB p65 and c-REL. Stimulation by inflammatory signals, such as TNFα, IL-1, and LPS, lead to the proteosomal degradation of IκBα and result in the translocation of RelA/NF-κB p65:p50 and c-Rel:p50 heterodimers to the nucleus. Subsequently, the nuclear NF-κB subunits bind to target genes and regulate gene transcription. NFKB1 is a key component of the NF-κB signaling pathway and is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, and immune responses.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse NF-κB p105/p50 recombinant protein (232-431 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500-1:5000 diluted (0.1 – 1 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The clone W16200A detects both p105 precursor protein and p50 subunit.

As per the Human Protein Atlas RNA level dataset, Molt-4 cell is expected to have a low level of NF-?B p105/p50 expression.

This clone does not work for IF microscopy application (in-house tested).

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2716155 (BioLegend Cat. No. 603901)
AB_2716155 (BioLegend Cat. No. 603902)

Antigen Details

Structure
968 amino acids with a predicted molecular weight of 105 kD. Contains an N-terminal RHD domain, 7 ankyrin repeats, and a C-terminal death domain. Can be processed to a 50kD p50 protein and a 70kD IκBγ protein.
Distribution

Cytoplasm. Translocates to the nucleus when activated.

Function
NFKB1 belongs to the NF-κB transcription factor family and participates in the regulation of cell proliferation, differentiation, apoptosis, and immune responses.
Interaction
NFKB1 p50 forms heterodimers with NF-κB p65 and c-Rel. Interacts with MAP3K8, DSIPI, SPAG9, UNC5CL, CFLAR, NFKBIE, NFKBIZ, NFKBID, MEN1, and HIF1AN.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Lougaris V, et al. 2017. Clin Immunol. 175:99.
2. Cartwright T, et al. 2016. FEBS J. 283:1812.
3. Li SK, et al. 2015. Mol Cell Biol. 35:1619.
4. Voce DJ, et al. 2015. Oncogene. 34:2807.
5. Chang PY, et al. 2006. Cancer Res. 66:6008.
6. Coleman TA, et al. 1993. Mol Cell Biol. 13:3850.

Gene ID
4790 View all products for this Gene ID
UniProt
View information about NF-kappaB p105/p50 on UniProt.org
Go To Top Version: 1    Revision Date: 09/29/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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