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Purified anti-NFATC4 Antibody

Pricing & Availability
Clone
W17026A (See other available formats)
Regulatory Status
RUO
Other Names
NFAT3, Nuclear factor of activated T-cells, cytoplasmic 4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17026A_PURE_NFATC4_Antibody_1_070519
Total cell lysates (15 µg total protein) from HepG2 and A549 cells (negative control), HEL and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-NFATC4 Antibody, clone W17026A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted NFATC4 expression data was obtained from Human Protein Atlas.
  • W17026A_PURE_NFATC4_Antibody_1_070519
    Total cell lysates (15 µg total protein) from HepG2 and A549 cells (negative control), HEL and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-NFATC4 Antibody, clone W17026A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted NFATC4 expression data was obtained from Human Protein Atlas.
  • W17026A_PURE_NFATC4_Antibody_2_070519
    Total cell lysates (15 µg total protein) from Daudi and SH-SY5Y cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-NFATC4 Antibody, clone W17026A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Check Availability Save
615401 25 µg $101
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615402 100 µg $253
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Description

NFATC4 is a transcriptional activator that induces expression of cytokine genes, including IL2 and IL4 in activated T cells. It also plays a central role in cardiovascular, muscle, and nervous cell development, differentiation, and function. Under basal conditions, NFATC4 is extensively phosphorylated and retained in the cytosol. Upon stimulation, elevated calcium results in NFATC4 dephosphorylation by calcineurin and subsequent translocation into the nucleus.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human NFATC4 protein corresponding to amino acids 887-902
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone displayed a weak affinity for mouse NFATC4 when tested by WB.

RRID
AB_2814456 (BioLegend Cat. No. 615401)
AB_2814456 (BioLegend Cat. No. 615402)

Antigen Details

Structure
The canonical isoform of NFATC4 is a 902 amino acid protein with a predicted molecular weight of 95 kD. Several isoforms of NFATC4 have been reported.
Distribution

Cells of the immune, nervous, and muscle system/nucleo-cytoplasmic localization

Function
Transcription factor
Cell Type
T cells
Biology Area
Transcription Factors
Antigen References
  1. Graef IA, et al. 2003. Cell. 113: 657.
  2. Crabtree GR, et al. 1999. Cell. 96:611.
  3. Lyakh L, et al. 1997. Mol Cell Biol. 17: 2475
Gene ID
4776 View all products for this Gene ID
UniProt
View information about NFATC4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/05/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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