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Purified anti-Notch1β Antibody

Pricing & Availability
Clone
5B6 (See other available formats)
Regulatory Status
RUO
Other Names
TAN1, Translocation-Associated Notch Protein TAN-1, Neurogenic Locus Notch Homolog Protein 1, hN1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
5B6_PURE_Notch1beta_Antibody_1_IHC_040716
IHC staining of clone 5B6 on formalin-fixed paraffin-embedded human stomach tissue at 1 µg/ml. Following Sodium Citrate antigen retrieval, the tissue was incubated with the primary antibody for 24 hours at 2-8°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection.
  • 5B6_PURE_Notch1beta_Antibody_1_IHC_040716
    IHC staining of clone 5B6 on formalin-fixed paraffin-embedded human stomach tissue at 1 µg/ml. Following Sodium Citrate antigen retrieval, the tissue was incubated with the primary antibody for 24 hours at 2-8°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection.
  • 5B6_PURE_Notch1beta_Antibody_2_WB_040716
    Western blot analysis of clone 5B6. Lane 1: 5 µg of HEK293 cell lysate overexpressing Notch1; Lane 2: 5 µg of control HEK293 cell lysate; Lane 3: 20 µg of tissue lysate from rat brain membrane fraction. The blot was incubated with the primary antibody at 10 µg/mL overnight at 4°C, followed by incubation with HRP goat anti-mouse (minimal x-reactivity) secondary antibody.
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845202 100 µg $265
 
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Description

Notch1 signaling is involved in tumorigenesis as well as in determining the fates of neural and non-neural cells during development and in adults. Alterations in the proteolysis of Notch1 by gamma-secretase could be involved in the pathogenesis of Alzheimer’s Disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH conjugated peptide corresponding to aa 1721-1745 of human Notch1 (VQSETVEPPPPAQLHFMYVAAAAFV)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Direct ELISA, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemcical staining. For immunohistochemistry, a concentration range of 1 - 10 µg/ml is recommended. For Direct ELISA, the suggested use of this reagent is 0.05 - 0.1 µg/ml. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 5B6 has been tested by Direct ELISA on Notch ß (Nß) peptide (1-17aa) coated plates. It has not been evaluated in a sandwich ELISA format.

RRID
AB_2616641 (BioLegend Cat. No. 845202)

Antigen Details

Structure
Notch1 is a 272 kD protein.
Distribution

Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart is observed.

Function
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell-fate determination. Affects the implementation of differentiation, proliferation and apoptotic programs.
Interaction
Notch1 is processed in a manner similar to APP, generating Nβ peptides (S4 cleavage) and the Notch intracellular domain (NICD, S3 cleavage) which translocates to the nucleus and acts as an essential regulator of transcription factors.
Ligand/Receptor
Receptor for the membrane ligands Jagged1, Jagged2, and Delta1 to regulate cell fate decisions.
Cell Type
Thymocytes
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References

1. Okochi M, et al. 2006. J. Biol. Chem. 281(12):7890.
2. Wanngren J, et al. 2012. J. Biol. Chem. 287(39):32640.
3. Tanveer R, et al. 2012. J. Biol. Chem. 287(41):34709.

Gene ID
4851 View all products for this Gene ID
UniProt
View information about Notch1beta on UniProt.org
Go To Top Version: 1    Revision Date: 04/13/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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