- Clone
- 5B6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TAN1, Translocation-Associated Notch Protein TAN-1, Neurogenic Locus Notch Homolog Protein 1, hN1
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![5B6_PURE_Notch1beta_Antibody_1_IHC_040716 5B6_PURE_Notch1beta_Antibody_1_IHC_040716](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/5B6_PURE_Notch1beta_Antibody_1_IHC_040716.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of clone 5B6 on formalin-fixed paraffin-embedded human stomach tissue at 1 µg/ml. Following Sodium Citrate antigen retrieval, the tissue was incubated with the primary antibody for 24 hours at 2-8°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection. -
Western blot analysis of clone 5B6. Lane 1: 5 µg of HEK293 cell lysate overexpressing Notch1; Lane 2: 5 µg of control HEK293 cell lysate; Lane 3: 20 µg of tissue lysate from rat brain membrane fraction. The blot was incubated with the primary antibody at 10 µg/mL overnight at 4°C, followed by incubation with HRP goat anti-mouse (minimal x-reactivity) secondary antibody.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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845202 | 100 µg | $265 |
Notch1 signaling is involved in tumorigenesis as well as in determining the fates of neural and non-neural cells during development and in adults. Alterations in the proteolysis of Notch1 by gamma-secretase could be involved in the pathogenesis of Alzheimer’s Disease.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH conjugated peptide corresponding to aa 1721-1745 of human Notch1 (VQSETVEPPPPAQLHFMYVAAAAFV)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
Direct ELISA, WB - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemcical staining. For immunohistochemistry, a concentration range of 1 - 10 µg/ml is recommended. For Direct ELISA, the suggested use of this reagent is 0.05 - 0.1 µg/ml. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Clone 5B6 has been tested by Direct ELISA on Notch ß (Nß) peptide (1-17aa) coated plates. It has not been evaluated in a sandwich ELISA format.
- RRID
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AB_2616641 (BioLegend Cat. No. 845202)
Antigen Details
- Structure
- Notch1 is a 272 kD protein.
- Distribution
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Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart is observed.
- Function
- Functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell-fate determination. Affects the implementation of differentiation, proliferation and apoptotic programs.
- Interaction
- Notch1 is processed in a manner similar to APP, generating Nβ peptides (S4 cleavage) and the Notch intracellular domain (NICD, S3 cleavage) which translocates to the nucleus and acts as an essential regulator of transcription factors.
- Ligand/Receptor
- Receptor for the membrane ligands Jagged1, Jagged2, and Delta1 to regulate cell fate decisions.
- Cell Type
- Thymocytes
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Antigen References
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1. Okochi M, et al. 2006. J. Biol. Chem. 281(12):7890.
2. Wanngren J, et al. 2012. J. Biol. Chem. 287(39):32640.
3. Tanveer R, et al. 2012. J. Biol. Chem. 287(41):34709. - Gene ID
- 4851 View all products for this Gene ID
- UniProt
- View information about Notch1beta on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Notch1β Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Notch1β | 5B6 | IHC-P,Direct ELISA,WB |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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