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Purified anti-Oligodendrocyte Marker O1 Antibody

Pricing & Availability
Clone
O1 (See other available formats)
Regulatory Status
RUO
Other Names
Galactocerebroside O1, Oligodendrocyte marker O1, Oligodendrocyte O1 antigen
Isotype
Mouse IgM, κ
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Product Citations
publications
O1_PURE_Oligodendrocyte-Marker-O1_Antibody_1_093033
IHC staining using purified anti-Oligodendrocyte Marker O1 (clone O1) on formalin-fixed paraffin-embedded human cerebellum tissue. The tissue was incubated overnight at 4°C with 0.5 μg/mL of anti-Oligodendrocyte Marker O1 (left) or without primary (right), followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgM (red) (Cat. No. 406526) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade mountant reagent. The image was captured with a 40X objective. Scalebar = 50 μm
  • O1_PURE_Oligodendrocyte-Marker-O1_Antibody_1_093033
    IHC staining using purified anti-Oligodendrocyte Marker O1 (clone O1) on formalin-fixed paraffin-embedded human cerebellum tissue. The tissue was incubated overnight at 4°C with 0.5 μg/mL of anti-Oligodendrocyte Marker O1 (left) or without primary (right), followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgM (red) (Cat. No. 406526) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade mountant reagent. The image was captured with a 40X objective. Scalebar = 50 μm
  • O1_PURE_Oligodendrocyte-Marker-O1_Antibody_2_093033
    Rat cortical stem cells fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:100 dilution (5.0 µg/mL) of purified anti-Oligodendrocyte Marker O1 (Clone O1) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective. Scale: 50 µm
  • O1_PURE_Oligodendrocyte-Marker-O1_Antibody_3_093033
    Differentiated rat cortical stem cells were fixed and permeabilized using Cyto-Fast Buffer (Cat. No. 426803) and stained with anti-Oligodendrocyte Marker O1 antibody (clone O1) (red dot plot), or mouse IgM, κ isotype control (blue dot plot) (Cat. No. 401602), followed by Alexa Fluor® 647 anti-mouse IgM (Cat. No. 406526). Left density plot (FSC-A vs. SSC-A) shows the gated population (oligodendrocytes and oligodendrocyte progenitor cells (OPC)), and right dot plots (Alexa Fluor® 647 vs. FSC-A) show stain intensity for each specific gated population.
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607751 25 µg $145
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Description

Oligodendrocytes are the myelinating cells of the central nervous system (CNS). Similar to the Schwann cells in the peripheral nervous system (PNS), their function is to provide support and isolation to neuronal axons. Their importance has been denoted on de-myelinating diseases such as multiple sclerosis, amyotrophic lateral sclerosis, periventricular leukomalacia, and also, they have been associated with dementia, schizophrenia, cognitive impairment, and bipolar disorder. Oligodendrocytes develop during embryogenesis; however, a small proportion of progenitor stem cells are present in the adult brain. Thus, three stages of differentiation can be identified in the cerebral white matter, and specific markers have been associated which each particular stage. Galactocerebroside O1 (marker O1) is one of the major galactosphingolipids components of oligodendrocytes plasma membrane. It is produced from late immature non-myelinating oligodendrocytes to mature myelinating oligodendrocytes, and together with expression of CNPase, it has been widely used as characteristic marker for these specific differentiation stages.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
White matter from bovine corpus callosum (from developer)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality testeed
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on testis samples, a concentration range of 1.25 - 5.0 µg/mL is suggested. For immunohistochemistry on cerebellum samples, a concentration range of 0.5 - 1.25 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone O1 recognize specifically O1 antigen by immunocytochemistry in oligodendrocytes derived from cortical stem cell using different fixation/permeabilization methods such as methanol, PFA/Triton X-100, and PFA/methanol, where the last method shows the highest intensity.

By immunohistochemistry in paraffin sections, clone O1 showed specific high staining on cerebellum white matter, and not staining in the molecular layer at the recommended concentration.

By flow cytometry staining on differentiated cortical stem cells, clone O1 stained at high intensity complex cells (oligodendrocytes) compared with low complexity cells (undifferentiated progenitor stem cells).

RRID
AB_2927903 (BioLegend Cat. No. 607751)
AB_2927903 (BioLegend Cat. No. 607752)

Antigen Details

Structure
It is a galactocerebroside (glycosphingolipid), composed of a single galactose linked to a ceramide
Distribution

Present in the plasma membrane of late immature to mature oligodendrocytes. 

Cell Type
Neural Stem Cells, Oligodendrocytes
Biology Area
Neurodegeneration, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Bansal R, et al. 1989. J Neurosci Res. 24:548-57.
  2. Bansal R, et al. 1999. J Neurosci. 19:7913-24.
  3. Ahmad I, et al. 2021. PloS One. 16:e0256155.
  4. Rasti BF, et al. 2020. Int J Nanomedicine. 15:3903-3920.
Gene ID
NA
UniProt
View information about Oligodendrocyte Marker O1 on UniProt.org

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Go To Top Version: 1    Revision Date: 09/30/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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