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Purified anti-Optineurin Antibody

Pricing & Availability
Clone
A18152B (See other available formats)
Regulatory Status
RUO
Other Names
E3-14.7K-interacting protein, FIP-2, Huntingtin yeast partner L, Huntingtin-interacting protein 7, HIP-7, NEMO-related protein, Optic neuropathy-inducing protein, Transcription factor IIIA-interacting protein, TFIIIA-IntP
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A18152B_PURE_Optineurin_Antibody_1_120219
Western blot of purified anti-Optineurin antibody (clone A18152B). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of A431 cell lysate; Lane 4: 20 µg of HeLa cell lysate (negative control). The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18152B_PURE_Optineurin_Antibody_1_120219
    Western blot of purified anti-Optineurin antibody (clone A18152B). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of A431 cell lysate; Lane 4: 20 µg of HeLa cell lysate (negative control). The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18152B_PURE_Optineurin_Antibody_2_120219
    ICC staining of purified anti-Optineurin antibody (clone A18152B) on A431 cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 60X objective. Scale bar: 50 µm
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870101 25 µg $106
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870102 100 µg $276
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Description

Optineurin (OPTN), also known as FIP-2 or NEMO-related protein (NRP), is a cytoplasmic protein associated with diverse cellular functions including membrane trafficking, vesicle transport, and cell cycle control. OPTN has also been identified as an autophagy receptor important in elimination of damaged mitochondria through mitophagy, a selective macroautophagy process. Mutations in the Optn gene have been linked to a variety of disorders including amyotrophic lateral sclerosis, glaucoma, and Paget's disease of bone. In vitro cellular studies suggest that optineurin recognizes various protein aggregates via its C-terminal coiled-coiled domain in a ubiquitin-independent manner and facilitates autophagic clearance of protein aggregates. Immunohistochemical analyses demonstrate the localization of OPTN to pathological tau tangles in Alzheimer's disease tissues, and Lewy bodies and neurites in Parkinson's disease tissues, supporting its role in neurodegenerative diseases.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human optineurin protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 2.0 - 10 µg/mL. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Duan K, et al. 2021. Neuron. 109:3793. PubMed
RRID
AB_2820187 (BioLegend Cat. No. 870101)
AB_2820187 (BioLegend Cat. No. 870102)

Antigen Details

Structure
Human optineurin is a 577 amino acid protein with a predicted molecular mass of 66 kD and an observed molecular mass of 74 kD.
Distribution

Tissue distribution: Widely expressed in tissues

Cellular distribution: Nucleus, endosome, cytosol, Golgi apparatus, mitochondrion, lysosome

Function
Optineurin plays an important role in the maintenance of the Golgi complex, membrane trafficking and exocytosis.
Interaction
Interacts with myosin VI and Rab8
Cell Type
Astrocytes, Neurons
Biology Area
Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Molecular Family
Adaptor Proteins
Antigen References
  1. Osawa T, et al. 2011. Neuropathology. 31:569-74.
  2. Ying H, Yue BY. 2012. Int. Rev. Cell. Mol. Biol. 294: 223-58. 
  3. Korac J, et al. 2013. J. Cell. Sci. 126: 580-92.
Gene ID
10133 View all products for this Gene ID
UniProt
View information about Optineurin on UniProt.org
Go To Top Version: 1    Revision Date: 12/02/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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