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Purified anti-p21 Antibody

Pricing & Availability
Clone
W22006E (See other available formats)
Regulatory Status
RUO
Other Names
Cyclin Dependent Kinase Inhibitor 1A, CAP20, CIP1, WAF1, SDI1, p21, p21 CIP1, CDKN1, p21 Cip1/Waf1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W22006E_PURE_p21_WB_072924
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and then probed with Purified anti-p21 (clone W22006E). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: molecular weight.
  • A. W22006E_PURE_p21_WB_072924
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and then probed with Purified anti-p21 (clone W22006E). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: molecular weight.
  • B. W22006E_PURE_p21_IHC-F_072924
    IHC staining with Purified anti-p21 on formalin-fixed paraffin-embedded human colon tissue was performed following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0). The tissue sections were incubated without (panel A) or with Purified anti- p21 (clone W22006E) (panels B (in 10X) and panel C (in 40X)) overnight at 4°C followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801). Scale Bar: 50 µm
  • C. W22006E_PURE_p21_ICC_072924
    Serum-starved A431 cells (negative control, top panels) and A549 cells (positive control, bottom panels) were fixed and permeabilized with 4% PFA and ice-cold methanol. They were then intracellularly stained with Purified anti-p21 (clone W22006E) (right panels) or Purified rat IgG2b, κ isotype control (Cat. No. 400602) (left panels). This was followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) (red). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue) and Alexa Fluor® 488 anti-Cytokeratin (clone C-11) (Cat. No. 628608) (green). Scale bar 50 µm.
  • D. W22006E_PURE_p21_ICFC_072924
    Serum starved MCF-7 cells (positive control, filled histogram), or A431 cells (negative control, open histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Purified anti-p21 (clone W22006E) or Purified rat IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for either cell line) (Cat. No. 400602) followed by Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416). The cells were co-stained with DAPI (Cat. No. 422801) for cell cycle analysis. Histogram represent cells gated on G1/G0.
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645951 25 µg $165
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645952 100 µg $490
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Description

p21 (also known as CIP1 or CDKN1A) is a G1 cell cycle regulator that inhibits cyclin-cyclin dependent kinase 2 and/or cyclin-cyclin dependent kinase 4 complexes. Its expression is highly controlled by the tumor suppressor protein p53, thus, p21 is involved in controlling the G1 cell cycle arrest in response to different stress stimuli. p21 plays a regulatory role in S phase DNA replication by interacting with PCNA (proliferating cell nuclear antigen). Activation of cyclin-dependent kinase 2 has been observed after p21 is cleaved by CASP3-like caspases. Loss of function of p21 has been associated with bladder cancer, Multiple Endocrine Neoplasia Type I and lymph node metastasis. In addition, p21 can bind the HIV integrase mediating resistance to infection in hematopoietic cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein fragment of p21
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use of this antibody (clone W22006E) in immunocytochemistry (ICC), we recommend fixation/permeabilization with either PFA/Triton X-100 or PFA/Methanol. This antibody is not suitable for use with methanol fixation/permeabilization.

RRID
AB_3662327 (BioLegend Cat. No. 645951)
AB_3662327 (BioLegend Cat. No. 645952)

Antigen Details

Structure
p21 is a 164 amino acid length protein with an approximate molecular weight of 18 kD.
Distribution

Nuclear localization; in some diseases, it is localized in the cytoplasm.

Function
Control of cell cycle progression
Inhibition of cyclin-dependent kinase activity
Inhibits DNA synthesis by competing with POLD3
Play a role in p53 inhibition of cell proliferation in response to DNA damage
Interaction
Nrf2, BCCIP, CIZ1, CUL4A, CCNE1, CDK, DDB1, DTL, GADD45A, GADD45G, HDAC, PCNA, PIM1, TK1, TSG101
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability
Molecular Family
Tumor Suppressors
Antigen References
  1. Sorteberg AL, et al. 2022. Front Oncol. 12:906194.
  2. Galanos P, et al. 2016. Cell Cycle. 23:3153-3154.
  3. Lai L, et al. 2020. Int J Mol Sci. 21:8504.
  4. Xiao BD, et al. 2020. World Stem Cells. 12:481.
Gene ID
1026 View all products for this Gene ID
UniProt
View information about p21 on UniProt.org

Other Formats

View All p21 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-p21 W22006E WB,IHC-P,ICC,ICFC
Go To Top Version: 1    Revision Date: 08/05/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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