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Purified anti-PGR Antibody

Pricing & Availability
Clone
BSB-2 (See other available formats)
Regulatory Status
RUO
Other Names
Progesterone receptor (PGR), nuclear receptor subfamily 3, group C, member 3 (NR3C3)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a. BSB-2_PURE_PGR_-IHC-P_032523
IHC staining of purified anti-PGR (clone BSB-2) on formalin-fixed paraffin-embedded human uterus tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 5 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 60X objective. Scale bar: 50 µm
  • a. BSB-2_PURE_PGR_-IHC-P_032523
    IHC staining of purified anti-PGR (clone BSB-2) on formalin-fixed paraffin-embedded human uterus tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 5 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 60X objective. Scale bar: 50 µm
  • b. BSB-2_PURE_PGR_-ICC-_032523
    MCF-7 cells were fixed with 4% Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then incubated with 1.25 µg/mL of either purified mouse IgG1, κ isotype control (Cat. No. 400102) (Panel A) or purified anti-PGR (clone BSB-2) (Panel B), followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 60X objective. Scale bar: 50 µm
  • BSB-2_PURE_PGR_-WB-_032523
    Whole cell extracts (15 µg total protein) from the indicated cell lines and tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and incubated with 1 µg/mL of purified anti-PGR (clone BSB-2) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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614751 25 µg $120
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614752 100 µg $395
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Description

PGR (Progesterone receptor) is a nuclear receptor, a type of receptor protein that directly interacts with DNA to affect gene expression upon ligand binding. PGR is activated by the hormone progesterone, a steroid and sex hormone. Widely expressed in female reproductive tissues, activation of PGR is critical for the establishment and maintenance of pregnancy and in the development of the mammary glands. PGR is also expressed in the central nervous system, modulating innervation by dopaminergic neurons. Overexpression of PGR is often found in breast, ovarian and uterine cancers, and PGR is one of the three major receptors assessed when classifying breast tumors. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human progesterone receptor
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration of 1.25 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product does not recognize mouse progesterone receptor in Western blot.

This product only recognizes PGR Isoform B.

For immunocytochemistry, fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 0.5% Triton-X is recommended. Fixation with Fixation Buffer followed by permeabilization with 100% methanol, or fixation with 100% methanol are not recommended.

RRID
AB_2941619 (BioLegend Cat. No. 614751)
AB_2941619 (BioLegend Cat. No. 614752)

Antigen Details

Structure
PGR has two major isoforms. Isoform A is a 769 amino acid protein with a predicted molecular weight of 82.3 kD. Isoform B is a 933 amino acid protein with a predicted molecular weight of 99.0 kD.
Distribution

Nucleus

Function
Nuclear receptor, transcription factor
Interaction
Upon ligand binding, PGR interacts directly with genomic DNA
Ligand/Receptor
Progesterone
Cell Type
Dopaminergic Neurons, Hematopoietic stem and progenitors, Neurons
Biology Area
Cell Cycle/DNA Replication, Neuroscience, Stem Cells, Transcription Factors
Molecular Family
Hormones, Steroid Receptors/Nuclear Receptors
Antigen References
  1. Grimm SL, et al. 2016. J Mol Biol. 428:3831-49.
  2. Yip CH, et al. 2014. Future Oncol. 10:2293-301.
  3. Cope DI and Mosivais D. 2022. Cells. 11:1474.
Gene ID
5241 View all products for this Gene ID
UniProt
View information about PGR on UniProt.org
Go To Top Version: 1    Revision Date: 03/24/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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