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Purified anti-PLK-1 Phospho (Thr210) Antibody

Pricing & Availability
Clone
2A3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Serine/Threonine protein kinase PLK, Polo-like kinase (PLK), Serine-threonine protein kinase 13
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2A3_PURE_PLK1-Phospho-Thr210_Antibody_1_120619
Whole cell extracts (15 µg protein) from serum starved HeLa untreated (-) or treated (+) with 200 ng/mL Nocodazole for 20 hrs were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. The membrane was treated (+ LPP) or untreated (- LPP) with lambda protein phosphatase, overnight at 4°C and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-PLK-1 Phospho (Thr210) Antibody, clone 2A3, 2hrs at RT. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Purified anti PLK-1 Antibody (Cat. No. 627702) was used as a pan PLK-1 loading control. Lane M: Molecular weight marker.
  • 2A3_PURE_PLK1-Phospho-Thr210_Antibody_1_120619
    Whole cell extracts (15 µg protein) from serum starved HeLa untreated (-) or treated (+) with 200 ng/mL Nocodazole for 20 hrs were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. The membrane was treated (+ LPP) or untreated (- LPP) with lambda protein phosphatase, overnight at 4°C and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-PLK-1 Phospho (Thr210) Antibody, clone 2A3, 2hrs at RT. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Purified anti PLK-1 Antibody (Cat. No. 627702) was used as a pan PLK-1 loading control. Lane M: Molecular weight marker.
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628901 25 µg $118
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628902 100 µg $275
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Description

PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The 2A3 antibody recognizes human phosphorylated PLK-1 (Thr210) and has been shown to be useful for Western blotting. To increase specificity, it is recommended that the 2A3 antibody be used for Western blotting after immunoprecipitation with the pan-specific PLK-1 3F8 antibody.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Modified peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Molli PR, et al. 2010. J Cell Biol. 190:101. PubMed
  2. Yang S, et al. 2013. Cancer Res. 73:6722. PubMed
Product Citations
  1. Pop OT, et al. 2023. Cell Mol Gastroenterol Hepatol. 17:16. PubMed
  2. Bucko PJ, et al. 2019. eLife. 0.333333333333333. PubMed
  3. Bucko PJ, et al. 2020. J Biol Chem. 295:13784. PubMed
  4. Yang S, et al. 2013. Cancer Res. 73:6722. PubMed
  5. Molli P, et al. 2010. J Cell Biol. 190:101. PubMed
  6. Tankiewicz‐Kwedlo A et al. 2017. British journal of pharmacology. 175(5):743-762 . PubMed
RRID
AB_439786 (BioLegend Cat. No. 628901)
AB_439786 (BioLegend Cat. No. 628902)

Antigen Details

Structure
Serine/Threonine family of protein kinases, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila). Contains two polo box domains. Predicted molecular weight 68 kD
Distribution

Nuclear protein, highly expressed in placenta and colon

Function
Regulates cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. May be required for cell division. Depletion of PLK-1 results in apoptosis
Interaction
Interacts with nuclear distribution gene C
Modification
Phosphorylation (Thr210 is major phosphorylation site in activated PLK-1 from mitotic cells)
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References

1. Hamanaka R, et al. 1994. Cell Growth Differ. 5:249.
2. Lake RJ, et al. 1993. Mol. Cell. Biol. 13:7793.
3. Holtrich U, et al. 1994. P. Natl. Acad. Sci. USA 91:1736.

Regulation
Upregulated by growth stimulating agents. Regulated by cell cycle position (highest in G2/M phase and declines to nearly undetectable levels after mitosis and throughout G1)
Gene ID
5347 View all products for this Gene ID
UniProt
View information about PLK-1 Phospho Thr210 on UniProt.org
Go To Top Version: 4    Revision Date: 12/06/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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