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Purified anti-PLZF Antibody

Pricing & Availability
Clone
W18031A (See other available formats)
Regulatory Status
RUO
Other Names
ZBTB16, Zinc Finger And BTB Domain Containing 16, ZNF145, Promyelocytic Leukaemia Zinc Finger
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
W18031A_PURE_PLZF_Antibody_071519.png
Total cell lysates (15 µg total protein) from A431, THP-1, and MOLT-4 (reduced expression controls), and HEL cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-PLZF Antibody, clone W18031A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower) Lane M: Molecular Weight marker. Predicted ZBTB16 expression data was obtained from Human Protein Atlas.
  • W18031A_PURE_PLZF_Antibody_071519.png
    Total cell lysates (15 µg total protein) from A431, THP-1, and MOLT-4 (reduced expression controls), and HEL cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-PLZF Antibody, clone W18031A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower) Lane M: Molecular Weight marker. Predicted ZBTB16 expression data was obtained from Human Protein Atlas.
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625601 25 µg $101
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625602 100 µg $253
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Description

Promyelocytic leukaemia zinc finger protein (PLZF) is a transcription factor that belongs to the POK (POZ and Krüppel) family of transcriptional repressors. It is involved in cell cycle control, and plays an essential role in limb development, differentiation of myeloid cells, and spermatogenesis. In certain acute myeloid leukemias, PLZF exists as a translocation product with Retinoic Acid Receptor-α (PLZF-RARα) that has a dominant negative effect on hematopoietic differentiation and is capable of activating Wnt signaling by upregulating the γ-catenin promoter. PLZF can also repress transcription of TP53 and also destabilizes p53 protein through enhanced ubiquitination.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human PLZF protein corresponding to amino acid residues 1-254
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has not been tested for ICC or IP.

RRID
AB_2814491 (BioLegend Cat. No. 625601)
AB_2814491 (BioLegend Cat. No. 625602)

Antigen Details

Structure
PLZF is a 673 amino acid protein with a predicted molecular weight of 74 kD.
Distribution

Nucleus/widely expressed

Function
Transcriptional repressor/epigenetic regulator
Cell Type
Mesenchymal Stem Cells, NKT cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction, Stem Cells, Transcription Factors
Antigen References
  1. Choi WI, et al. 2014. J. Biol. Chem. 289: 18625.
  2. Constantinides MG, et al. 2011. J. Immunol. 187:309.
  3. Kovalovsky D, et al. 2008. Nat. Immunol. 9:1055.
  4. Zheng X, et al. 2004. Blood. 103:3535.
  5. Chen Z, et al. 1994. Proc. Natl. Acad. Sci. USA 91:1178.
  6. Chen Z, et al. 1993. EMBO J. 12:1161.

 

Gene ID
7704 View all products for this Gene ID
UniProt
View information about PLZF on UniProt.org
Go To Top Version: 1    Revision Date: 07/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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