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Purified anti-PPARγ Antibody

Pricing & Availability
Clone
14G4B17 (See other available formats)
Regulatory Status
RUO
Other Names
Peroxisome proliferator-activated receptor gamma, PPAR gamma, NR1C3
Isotype
Mouse IgG1, κ
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Product Citations
publications
14G4B17_PURE_PPARgamma_Antibody_1_WB_031516
Total cell lysate from mouse 3T3-L1 cells (lane 1), differentiated 3T3-L1 cells (lane 2), human PC3 cells (lane 3), and human THP-1 cells (lane 4) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human/mouse PPARγ (clone 14G4B17) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG (minimal x-reactivity) Antibody and chemiluminescence detection.
  • 14G4B17_PURE_PPARgamma_Antibody_1_WB_031516
    Total cell lysate from mouse 3T3-L1 cells (lane 1), differentiated 3T3-L1 cells (lane 2), human PC3 cells (lane 3), and human THP-1 cells (lane 4) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human/mouse PPARγ (clone 14G4B17) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG (minimal x-reactivity) Antibody and chemiluminescence detection.
  • 14G4B17_PURE_PPARgamma_Antibody_2_IF_031516
    PC3 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/ml anti-human/mouse PPARγ (clone 14G4B17) antibody for two hours followed by Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity) Antibody (red) for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured by a 60X objective.
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683402 100 µg $288
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Description

PPARγ, also known as NR1C3, is a ligand-dependent transcription factor belonging to the peroxisome-proliferator activated receptor (PPAR) family. Upon activation by its ligands, such as fatty acids and prostaglandin PGJ2, PPARγ forms heterodimers with the retinoid X receptors (RXRs) and binds to DNA specific PPAR response elements (PPRE). In the absence of ligand, the PPARγ/RXR heterodimer represses its target genes by recruiting and stabilizing the corepressor complexes at the promoter regions. PPARγ is expressed most abundantly in adipose tissue and induced during adipocyte differentiation. PPARγ has been suggested to regulate the expression of key genes involved in glucose metabolism, lipid storage, energy homeostasis, adipogenesis, atherosclerosis, inflammation, and cancer.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic PPARγ peptide conjugated to KLH (CETDMSLHPLLQEIYKDLY)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
RRID
AB_2572020 (BioLegend Cat. No. 683402)

Antigen Details

Structure
505 amino acids with an approximate molecular weight of 57.6 kD. Contains two zinc finger domains responsible for DNA binding.
Distribution

Nucleus.

Function
PPARγ is a nuclear receptor that modulates transcription of its target genes. It plays crucial roles in inflammation, energy homeostasis, and adipocyte differentiation.
Interaction
PPARγ interacts with FOXO1, RXRA, PPARBP, ASXL1, ASXL2, DNTTIP2, FAM120B, MAP2K1/MEK1, NR0B2, PDPK1, PRDM16, PRMT2 and TGFB1I1.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Majithia AR, et al. 2014. Proc. Natl. Acad. Sci. USA 111:13127.
2. Aprile M, et al. 2014. PPAR. Res. 2014:537865.
3. Pott S, et al. 2013. PLoS One 7:e48102.
4. Klemke M, et al. 2011. Cancer Genet. 204:334.
5. Folwaczny M, et al. 2011. Innate Immun. 17:541.
6. Fuentes P, et al. 2010. J. Endocrinol. 206:75.

Gene ID
5468 View all products for this Gene ID
UniProt
View information about PPARgamma on UniProt.org
Go To Top Version: 1    Revision Date: 03/18/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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