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Purified anti-PPP1CA Antibody

Pricing & Availability
Clone
W22109B (See other available formats)
Regulatory Status
RUO
Other Names
PP1A, PP-1A, PPP1A, PP1alpha, PP1α
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W22109B_PURE_PPP1CA_Antibody_1_010824
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with the 1 µg/mL of Purified anti-PPP1CA (clone W22109B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W22109B_PURE_PPP1CA_Antibody_1_010824
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with the 1 µg/mL of Purified anti-PPP1CA (clone W22109B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W22109B_PURE_PPP1CA_Antibody_2_010824
    MCF7 cells were fixed with ice-cold methanol and blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 5 µg/mL of Purified anti-PPP1CA (clone W22109B), followed by incubation with 2.5 µg/mL of Alexa Fluor® 488 Goat anti-rat IgG (Cat. No. 405418) for 1 hour at room temperature (green, panel A). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue, panel B). The image was captured with a 40X objective. Scale bar: 25 µm
  • W22109B_PURE_PPP1CA_Antibody_3_010824
    HeLa cells (mock) (panels A and B) and HeLa PPP1CA knockdown cells (panels C and D) were fixed with fixation buffer (Cat. No. 420801), permeabilized with 0.5% Triton, and then blocked with 5% FBS for 1 hour at room temperature. Cells were intracellularly stained with 1 µg/mL of Purified anti-PPP1CA (clone W22109B), followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) (red) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue). The image was captured with a 40X objective. Scale bar: 25 µm
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621951 25 µg $165
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621952 100 µg $415
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Description

Protein phosphatase 1 catalytic subunit alpha (PPP1CA) is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine-specific protein phosphatase involved in the regulation of various cellular processes, such as cell division, glycogen metabolism, muscle contraction, protein synthesis, regulation of membrane receptors and channels, and HIV-1 virus transcription. PP1 has been shown to dephosphorylate RB and CDC25 during mitosis. Cell cycle-dependent phosphorylation at Thr320 of PPP1CA by CDC2 kinase inhibits PPP1CA activity. The studies in human and mice indicate that PP1 is an important regulator of cardiac function, and also show that PP1 suppresses learning and memory in mice.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
A synthetic peptide of human PPP1CA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The clone cross-reacts with mouse and rat in western blotting (WB).

For immunocytochemistry (ICC), we recommend to use 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice cold-methanol.

This product is not recommended for use in immunohistochemistry (IHC).

RRID
AB_3097497 (BioLegend Cat. No. 621951)
AB_3097497 (BioLegend Cat. No. 621952)

Antigen Details

Structure
PPP1CA protein is a 330 amino acid protein with a predicted molecular weight of 38 kD
Distribution

Upregulated in synovial fluid mononuclear cells and peripheral blood mononuclear cells from patients with rheumatoid arthritis

Interaction
FOXP3, CENPA, ATG16L1, TNS1, SAXO4, PPP1R21, PPP1R26, PPP1R27, PPP1R35, PPP1R36, PPP1R37, SH3RF2, ELFN1 and ELFN2
Cell Type
Neurons, Tregs
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Neuroscience, Protein Synthesis
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Rubin E, et al.1998. Front Biosci. 3:D1209-19.
  2. Durfee T, et al. 1993. Genes Dev. 7:555-69.
  3. Izumi T, et al. 1992. Mol Biol Cell. 3:927-39.
  4. Kwon YG, et al. 1997. PNAS. 94:2168-73.
  5. Carr AN, et al. 2002. Mol Cell Biol. 22:4124-35.
  6. Genoux D, et al. 2002. Nature. 418:970-5.
Gene ID
5499 View all products for this Gene ID
UniProt
View information about PPP1CA on UniProt.org
Go To Top Version: 1    Revision Date: 01/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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