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Purified anti-PPP2R2A Antibody (Previously Covance catalog# MMS-5099)

Pricing & Availability
Clone
2G9 (See other available formats)
Regulatory Status
RUO
Other Names
Serine/threonine-protein phosphatase 2A 55 kD regulatory subunit B alpha isoform, B55A, B55ALPHA, PR52A, PR55A, protein phosphatase 2 (formerly 2A), regulatory subunit B (PR52)
Previously
Covance Catalog# MMS-5099
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
2G9_Purified_PPP2R2A_antibody_1_102218
Western blot of purified anti-PPP2R2A antibody (clone 2G9). Lane 1: Molecular weight marker; Lane 2: 20 µg of 3T3 cell lysate; Lane 3: 20 µg of human brain lysate; Lane 4: 20 µg of mouse brain lysate; Lane 5: 20 µg of rat brain lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2G9_Purified_PPP2R2A_antibody_1_102218
    Western blot of purified anti-PPP2R2A antibody (clone 2G9). Lane 1: Molecular weight marker; Lane 2: 20 µg of 3T3 cell lysate; Lane 3: 20 µg of human brain lysate; Lane 4: 20 µg of mouse brain lysate; Lane 5: 20 µg of rat brain lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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831301 100 µL $275
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Description

Protein phosphatase 2A is a heterotrimeric protein serine/threonine phosphatase consisting of a 36-kD catalytic C subunit (alpha and beta isoforms), a 65-kD structural A subunit (alpha and beta isoforms), and a variable regulatory B subunit. When the PP2A catalytic C subunit associates with the A and B subunits several species of holoenzymes are produced with distinct functions and characteristics. Ubiquitously expressed, PP2A is involved in a broad range of cellular processes, including membrane receptor desensitization, signal transduction, intermediary metabolism, transcriptional regulation and control of DNA replication, and mitosis.

The PP2A phosphatase family accounts for the majority of Ser/Thr phosphatase activity in the brain and specifically regulates tau phosphorylation, both directly as tau phosphatase and indirectly by regulating the activities of several tau kinases which include CaM Kinase II, PKA, MAP kinase kinase (MEK 1/2), extracellular regulated kinase (ERK 1/2), GSK-3β, and P70S6 kinase, thus regulating tau microtubule binding in the brain.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a synthetic peptide corresponding to residues surrounding Asp406 of rat PP2R2A/PP2A, B55-α/PR55-α protein.
Formulation
Phosphate-buffered solution with BSA + 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Sontag E, et al. 2004. J Neuropathol Exp Neurol. 63:287. (WB, IHC-P)
RRID
AB_2564946 (BioLegend Cat. No. 831301)

Antigen Details

Structure
Expected MW: 55 kD
Cell Type
Neurons
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation
Molecular Family
Protein Kinases/Phosphatase
Gene ID
5520 View all products for this Gene ID
UniProt
View information about PPP2R2A on UniProt.org
Go To Top Version: 4    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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