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- Clone
- O91F10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PPT1, Palmitoyl-Protein Thioesterase 1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of purified anti-PPT1 antibody (clone O91F10). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 855801 | 25 µg | $106 | ||||
| 855802 | 100 µg | $276 | ||||
PPT1 (Palmitoyl-protein thioesterase-1) was originally discovered as the enzyme that catalyzes polypeptide depalmitoylation. During lysosomal degradation, PPT1 removes the thioester-linked palmitic acid from the modified cysteine of targeted protein, which is important to palmitoylated protein degradation. Deficits in PPT1 have been identified as the leading cause of infantile neuronal ceroid liposuscinosis. Recent research has also linked PPT1 to Huntington Disease, Alzheimer disease, Schizophrenia, and mental retardation, as S-palmitoylation has been found to affect synaptic regulation by regulating AMPA receptors trafficking and function.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein corresponding to amino acids 100-306 of human PPT1.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2783442 (BioLegend Cat. No. 855801)
AB_2783442 (BioLegend Cat. No. 855802)
Antigen Details
- Structure
- PPT1 is a 306 amino acid protein with an apparent molecular mass of ~34 kD.
- Distribution
-
Tissue Distribution: Ubiquitously expressed.
Cellular Distribution: Extracellular, nucleus, lysosome, golgi apparatus, and cytosol.
- Function
- PPT1 is involved in protein depalmitoylation and lysosomal degradation of palmitoylated protein.
- Interaction
- PPT1 interacts with CLN5, ATP5F1A, and ATP5F1B.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance, Synaptic Biology
- Molecular Family
- Presynaptic proteins
- Antigen References
-
- Han J, et al. 2015. Acta. Pharm. Sin. B. 5:1-7.
- Hawkins-Salsbury JA, et al. 2013. Biochim. Biophys. Acta. 11:1906-9.
- Young FB, et al. 2012. Prog. Neurobiol. 97:220.
- Gene ID
- 5538 View all products for this Gene ID
- UniProt
- View information about PPT1 on UniProt.org
Other Formats
View All PPT1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-PPT1 | O91F10 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-PPT1
Western blot of purified anti-PPT1 antibody (clone O91F10)....
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