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Purified anti-PRC1 Antibody

Pricing & Availability
Clone
6G2 (See other available formats)
Regulatory Status
RUO
Other Names
Protein regulator of cytokinesis 1, Mitotic spindle-associated CDK substrate, Protein regulating cytokinesis 1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
6G2_PURE_PRC1_Antibody_WB_050318
Total lysates (15 µg protein) from 293T and 293T/PRC1 CRISPR/Cas9 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-PRC1 antibody, clone 6G2 (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • 6G2_PURE_PRC1_Antibody_WB_050318
    Total lysates (15 µg protein) from 293T and 293T/PRC1 CRISPR/Cas9 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-PRC1 antibody, clone 6G2 (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
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629001 25 µg $106
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629002 100 µg $229
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Description

PRC1 (also known as protein regulator of cytokinesis 1 and mitotic spindle-associated CDK substrate) is a member of the MAP65/ASE1 family with a molecular weight of approximately 72 kD. PRC1 localization changes with cell-cycle position and can be found in the nucleus, the mitotic spindle, and the cell mid-body. PRC1 is a mitotic spindle-associated CDK substrate protein required for cytokinesis and maintenance of the spindle midzone. PRC1 can be modified by phosphorylation and has been shown to interact with Cdc2 and Cdk2. The 6G2 antibody recognizes the human PRC1 protein and has been shown to be useful for Western blotting.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino Acid: 1-150
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.5 - 5.0 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Neahring L, et al. 2021. Dev Cell. 56:3006. PubMed
  2. Elting MW et al. 2017. Current biology : CB. 27(14):2112-2122 . PubMed
  3. Udy D, et al. 2015. PLoS One. 10: 0134738. PubMed
  4. Suresh P, et al. 2020. eLife. 9:e53807.. PubMed
RRID
AB_2169532 (BioLegend Cat. No. 629001)
AB_2169532 (BioLegend Cat. No. 629002)

Antigen Details

Structure
Human PRC1 has 4 isoforms with MW ranging from 61-72 kD.
Distribution

Cell cycle-dependent; nuclear, mitotic spindle, cell mid-body

Function
Mitotic spindle-associated CDK substrate protein required for cytokinesis, maintains the spindle midzone
Interaction
Cdc2, Cdk2
Modification
Phosphorylation
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure
Antigen References

1. Jiang W, et al. 1998. Mol. Cell 2:877.
2. Mollinari C, et al. 2002. J. Cell Biol. 157:1175.

Gene ID
9055 View all products for this Gene ID
UniProt
View information about PRC1 on UniProt.org
Go To Top Version: 3    Revision Date: 05/03/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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