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Purified anti-Presenilin 1 (NH2-terminus) Antibody (Previously Covance catalog# PRB-354P)

Pricing & Availability
Clone
Poly18111 (See other available formats)
Regulatory Status
RUO
Other Names
PSEN1, AD3, Alzheimer disease 3, FAD, PS-1, PS1, S182, gamma-secretase
Previously
Covance Catalog# PRB-354P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
811101 100 µL $375
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Description

The gamma secretase is a multiprotein complex consisting of presenilin, nicastrin, Aph-1 and Pen-2. All four proteins are necessary for full proteolytic activity. The gamma secretase complex catalyzes cleavage of a number of integral membrane proteins, which include the Amyloid precursor protein, Notch, Erb4, and E-cadherin. The results of several recent studies indicate that the functional gamma secretase activity mostly arises from a low abundance and very high molecular weight complex (>670 kD). The data suggests that additional proteins may be present in these functional complexes or that the functional complex is a higher order multimer of presenilin, aph-1, pen-2, and nicastrin.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This polyclonal antibody was raised against a peptide corresponding to the N-terminus of human PS1.
Molecular Mass
Expected MW 50kD and 30kD
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by antigen affinity.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, ICC, IP
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
• Western blot: 1:1,000*

Application Notes

This antibody is effective in immunoblotting (WB).

WB Positive Control: Human Brain lysate


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Gu Y, Sanjo N, Chen F, Hasegawa H, Petit A, Ruan X, Li W, Shier C, Kawarai T, Schmitt-Ulms G, Westaway D, St George-Hyslop P, Fraser PE. The presenilin proteins are components of multiple membrane-bound complexes which have different biological activities. J Biol Chem 279(30):31329-31336, 2004.
  2. Yu G, Chen F, Nishimura M, Steiner H, Tandon A, Kawarai T, Arawaka S, Supala A, Song YQ, Rogaeva E, Holmes E, Zhang DM, Milman P, Fraser PE, Haass C, George-Hyslop PS. Mutation of conserved aspartates affects maturation of both aspartate mutant and endogenous presenilin1 and presenilin 2 complexes. J Biol Chem 275(35):27348-27353, 2000.
  3. Chen F, Yang DS, Petanceska S, Yang A, Tandon A, Yu G, Rozmahel R, Ghiso J, Nishimura M, Zhang DM, Kawarai T, Levesque G, Mills J, Levesque L, Song YQ, Rogaeva E, Westaway D, Mount H, Gandy S, St George-Hyslop P, Fraser PE. Carboxyl-terminal fragments of alzheimer beta-amyloid precursor protein accumulate in restricted and unpredicted intracellular compartments in presenilin 1-deficient cells. J Biol Chem 275(47):36794-36802, 2000.
Product Citations
  1. Lu J, et al. 2016. PLoS One. 11:e0166415. PubMed
  2. Tambini MD, et al. 2020. J Biol Chem. 295:7442. PubMed
RRID
AB_2564752 (BioLegend Cat. No. 811101)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Secretases
Gene ID
5663 View all products for this Gene ID
UniProt
View information about Presenilin 1 on UniProt.org
Go To Top Version: 3    Revision Date: 02/09/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-Presenilin 1 (NH2-terminus)

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