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Purified anti-Profilin-1 Antibody

Pricing & Availability
Clone
A18224E (See other available formats)
Regulatory Status
RUO
Other Names
Profilin 1, PFN1, Profilin1, Epididymis tissue protein Li 184a
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A_A18224E_PURE_Profilin-1_Antibody_WB_012624
Whole cell extracts (15 µg total protein) from the indicated cell lines/tissues were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL of Purified anti-Profilin-1 (clone A18224E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426316) was used as a detection agent. Lane M: Molecular weight marker
  • A_A18224E_PURE_Profilin-1_Antibody_WB_012624
    Whole cell extracts (15 µg total protein) from the indicated cell lines/tissues were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL of Purified anti-Profilin-1 (clone A18224E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426316) was used as a detection agent. Lane M: Molecular weight marker
  • B_A18224E_PURE_Profilin-1_Antibody_IHC_012624
    IHC staining of Purified anti-Profilin-1 (clone A18224E) on formalin-fixed paraffin-embedded human tonsil. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 10 μg/mL of Purified anti-Profilin-1 (clone A18224E) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 μm
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631751 25 µg $170
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631752 100 µg $405
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Description

Profilin-1 is an actin-binding protein responsible for the regulation of actin dynamics. Profilin-1 assists in the exchange of ADP for ATP on G-actin, facilitating the polymerization of actin monomers into filaments by promoting assembly at the growing end. Thus, profilin-1 is implicated in processes like cell motility, cytokinesis, and endocytosis, highlighting its significance in maintaining cellular structure and function. Profilin-1 also interacts with numerous proteins involved in signaling pathways, influencing cellular responses to intracellular and extracellular cues. Emerging evidence also shows that profilin-1 can bind to microtubules, facilitating interactions between the two major components of the cytoskeleton. Mutations in profilin-1 have been associated with amyotrophic lateral sclerosis (ALS) and is suspected to cause insoluble aggregates due to disruption in axonal outgrowth, cell integrity and protein distribution.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of the N terminus of human Profilin 1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody (clone A18224E) is not suitable for immunocytochemistry (ICC).

For immunohistochemistry (IHC), we recommend antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0). Antigen retrieval with Sodium Citrate is not recommended as it results in mis-localized signal.

RRID
AB_3097566 (BioLegend Cat. No. 631751)
AB_3097566 (BioLegend Cat. No. 631752)

Antigen Details

Structure
Profilin-1 is an 140 amino-acid protein with a predicted molecular weight of 15 kD.
Distribution

Ubiquitous, cytoskeleton/cytoplasm

Function
Actin dynamics, cell shape and motility
Interaction
Actin, Ena/VASP proteins, phosphoinositides, ROCK, RAP1, CDC42
Ligand/Receptor
Profilin-1 binds actin monomers
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure
Molecular Family
Microfilaments
Antigen References
  1. Murk K, et al. 2021. Front Cell Dev Biol. 9:681122.
  2. Karlsson R, et al. 2021. J Cell Physiol. 236:7256-7265.
  3. Davey RJ, et al. 2020. Biophys Rev. 12:827-849.
Gene ID
5216 View all products for this Gene ID
UniProt
View information about Profilin-1 on UniProt.org
Go To Top Version: 1    Revision Date: 01/26/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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