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Purified anti-rat CD38 Antibody

Pricing & Availability
Clone
14.27 (See other available formats)
Regulatory Status
RUO
Other Names
T10 , ADP-ribosyl cyclase
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
14.27_Purified_CD38_Antibody_FC_090513.jpg
Lou rat splenocytes were stained with purified anti-rat CD38 (clone 14.27, filled histogram), or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • 14.27_Purified_CD38_Antibody_FC_090513.jpg
    Lou rat splenocytes were stained with purified anti-rat CD38 (clone 14.27, filled histogram), or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • 14dot27_PURE_CD38_Antibody_2_010419
    Rat frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of CD3 (clone 1F4) Alexa Fluor® 488 (green), 10 µg/mL of CD11b/c (clone OX-42) Alexa Fluor® 647 (blue), and 10 µg/mL of purified CD38 (clone 14.27) overnight at 4°C, followed by 5 µg/mL of anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 594 (red) for two hours at room temperature. The image was captured by 10X objective.
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250502 100 µg $194
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Description

CD38 is a 45 kD type II transmembrane ADP-ribosyl cyclase expressed on early hematopoietic precursors and leukocytes, hepatic stellate cells, astrocytes and epithelial cells. CD38 expression can be upregulated upon cell activation. CD38 catalyzes the production of cyclic APD ribose, which is important in Ca2+ release. CD38 induces production of proinflammatory cytokines and proliferation. The ligand for CD38 is CD31. Impairment in CD38 expression is associated with immunological and behavioral disorders.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunohistochemistry staining on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry1 and Western blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. March S, et al. 2007. Am. J. Pathol. 170:176. (IHC, WB)
Product Citations
  1. Ardiana M, et al. 2022. F1000Res. 10:917. PubMed
  2. Boslett J, et al. 2018. Am J Physiol Cell Physiol. 314:C297. PubMed
RRID
AB_2562587 (BioLegend Cat. No. 250502)

Antigen Details

Structure
CD38 is a 45 kD type II transmembrane protein.
Distribution

Early hematopoietic precursors and leukocytes, astrocytes, hepatic stellate cells, and epithelial cells; upregulated upon activation.

Function
CD38 catalyzes the production of cyclic APD ribose, which is important in Ca2+ release. CD38 induces production of proinflammatory cytokines and proliferation.
Ligand/Receptor
CD31, hyaluronic acid
Cell Type
Astrocytes, B cells, Dendritic cells, Epithelial cells, Leukocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, MHC Antigens
Antigen References

1. Ota H, et al. 2012. Life Sci. 90:206.
2. Masuda W, et al. 2011. Arch. Biochem. Biophys. 2:131.
3. Khoo KM, et al. 1998. Biochem. Mol. Biol. Int. 44:841.
4. Prasad GS, et al. 1996. Nat. Struct. Biol. 3:957.

Gene ID
25668 View all products for this Gene ID
UniProt
View information about CD38 on UniProt.org
Go To Top Version: 2    Revision Date: 01/04/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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