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Purified anti-RelB Antibody

Pricing & Availability
Clone
W16195A (See other available formats)
Regulatory Status
RUO
Other Names
IREL, IMD53
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16195A_PURE_RelB_Antibody_1_082619
Whole cell extracts (15 µg total protein) from HeLa (reduced expression control), Daudi, Ramos, and Ba/F3 cells were resolved by 4-12% Tris-Glycine gel electrophoresis, transferred to a nitrocelullose membrane, and probed with 2.0 µg/mL (1:250 dilution) of Purified anti-RelB Antibody, clone W16195A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • W16195A_PURE_RelB_Antibody_1_082619
    Whole cell extracts (15 µg total protein) from HeLa (reduced expression control), Daudi, Ramos, and Ba/F3 cells were resolved by 4-12% Tris-Glycine gel electrophoresis, transferred to a nitrocelullose membrane, and probed with 2.0 µg/mL (1:250 dilution) of Purified anti-RelB Antibody, clone W16195A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
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615101 25 µg $101
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615102 100 µg $253
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Description

The Nuclear Factor kappa-light chain-enhancer of activated B cell, or NF- κB, is a transcriptional complex that plays a critical role in inducing expression of genes involved in inflammation, stress responses, cell growth, and apoptosis. The functional NF- κB complex can be comprised of homo- or heterodimeric units of Rel-like/homology domain containing proteins, including RelA, RelB, cRel, p50 and p52. RelB, which preferentially forms heterodimers with p50 and p52, is associated with non-canonical NF- κB signaling activation. This branch of NF- κB signaling is characterized by the NF-κB-inducing kinase (NIK)-linked processing of p100 instead of degradation of IκBα. Non-canonical NF-κB signaling is crucial for B-cell survival and maturation, and overexpression of RelB is associated with lymphoid malignancies and colorectal tumorigenesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human RelB protein corresponding to amino acid residues 69-308
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone may produce a ~65 kD non-speccific band by WB when used at higher concentrations.

This clone does not recognize mouse RelB.

RRID
AB_2814454 (BioLegend Cat. No. 615101)
AB_2814454 (BioLegend Cat. No. 615102)

Antigen Details

Structure
RelB is a 579 amino acid protein with a predicted molecular weight of 62 kD.
Distribution

Ubiquitously expressed; nuclear and cytosolic localization.

Function
Transcriptonal regulator
Cell Type
Hematopoietic stem and progenitors
Biology Area
Transcription Factors
Antigen References
  1. Beg AA, et al. 1992. Genes Dev. 6: 1899
  2. Lernbecher T, et al. 1993. Nature. 365: 767.
  3. Lernbecher T, et al. 1994. EMBO J. 13:4060.
  4. Sun SC. 2011. Cell Res. 21: 71
  5. Tao Y, et al. 2018. Ongogene. 37:5887
Gene ID
5971 View all products for this Gene ID
UniProt
View information about RelB on UniProt.org
Go To Top Version: 1    Revision Date: 08/26/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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