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Purified anti-RNA Polymerase II RPB5 Antibody

Pricing & Availability
Clone
3Y11 (See other available formats)
Regulatory Status
RUO
Other Names
DNA-directed RNA polymerases I, II, and III subunit RPABC1, PolR2E, RNA polymerases I, II, and III subunit ABC1, RPA7, RPC9
Isotype
Mouse IgG1, κ
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Product Citations
publications
3Y11_PURE_RNAPolymeraseII_RPB5_Antibody_082620
Whole cell lysates (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a 1.0 µg/mL (1:500 dilution) of purified anti-RNA Polymerase II RPB5 antibody (clone 3Y11) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Ponceau S solution was used to show protein loading (lower). Lane M: Molecular weight marker.
  • 3Y11_PURE_RNAPolymeraseII_RPB5_Antibody_082620
    Whole cell lysates (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a 1.0 µg/mL (1:500 dilution) of purified anti-RNA Polymerase II RPB5 antibody (clone 3Y11) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Ponceau S solution was used to show protein loading (lower). Lane M: Molecular weight marker.
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665205 25 µg $101
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665206 100 µg $253
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Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The common components of RNA polymerases I, II, and III synthesize ribosomal RNA precursors, mRNA precursors, and many functional non-coding RNAs and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, RPB5 is part of the lower jaw that surrounds the central large cleft and is believed to grab the incoming DNA template. It seems to be the major component in this process.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Yeast
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Previously item# W0014 from Neoclone.

RRID
AB_2876742 (BioLegend Cat. No. 665205)
AB_2876742 (BioLegend Cat. No. 665206)

Antigen Details

Structure
RPB5 is a 27 kD subunit of yeast RNA polymerase II.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
852451 View all products for this Gene ID
UniProt
View information about RNA Polymerase II RPB5 on UniProt.org
Go To Top Version: 2    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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