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- Clone
- A18156E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Polyubiquitin-C, Polyubiquitin B, RPS 27A, RPS27A, UBA 52, UBA 80, UBA52, UBA80, UBB, UBC, UBCEP 1, UBCEP 2, UBCEP1, UBCEP2, Ubiquitin, ubiquitin B
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
IHC staining of purified anti-RPS27A (clone A18156E) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using sodium citrate (Cat. No. 420902), the tissue was incubated without (panel A) and with (panel B) 5 μg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (magenta) (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 μm -
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-RPS27A (clone A18156E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 614451 | 25 µg | $135 | ||||
| 614452 | 100 µg | $410 | ||||
Ubiquitin is a small (8.5 kD) regulatory protein that is ubiquitously expressed in tissues of eukaryotic organisms. There are four genes in the human genome that produce ubiquitin: UBB, UBC, UBA52, and RPS27A. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. The UBB and UBC genes code for polyubiquitin precursor proteins.
Ubiquitination is a post-translational modification where a ubiquitin subunit is attached to a protein. Addition of ubiquitin can signal for degradation via the proteasome, alter cellular location, promote or prevent protein interactions, or affect activity. Ubiquitination is carried out stepwise by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. The cascade results in the binding of ubiquitin to lysine residues on the protein substrate via an isopeptide bond, cysteine residues through a thioester bond, serine and threonine residues through an ester bond, or the amino group of the protein's N-terminus via a peptide bond.
Proteins can be modified either by a single ubiquitin unit (monoubiquitination) or a chain of ubiquitin molecules (polyubiquitination). Proteins destined for degradation by the proteasome are usually ubiquitinated on lysine residues K48 and K29, while other polyubiquitinations (e.g. on K63, K11, K6) and monoubiquitinations may regulate processes such as endocytic trafficking, inflammation, translation and DNA repair.
A frameshift mutation in ubiquitin B can result in a truncated peptide missing the C-terminal glycine. This abnormal peptide, known as UBB+1, has been shown to accumulate selectively in Alzheimer's disease and other tauopathies.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant fragment of human RPS27A
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
WB - Verified
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product is not recommended for use in ICC. For immunohistochemistry, antigen retrieval with sodium citrate is recommended. Clone A18156E is equivalent to BioLegend’s clone P4G7 which detects ubiquitin and ubiquitinylated proteins. The ubiquitin protein is extremely well conserved and thus the antibodies have extensive species cross-reactivity from yeast to human.
- RRID
-
AB_3068138 (BioLegend Cat. No. 614451)
AB_3068138 (BioLegend Cat. No. 614452)
Antigen Details
- Structure
- RPS27A is a 156 amino acid S27a ribosomal protein fused with a single copy of ubiquitin.
- Distribution
-
Found in almost all tissues of eukaryotic organisms
- Function
- Regulatory protein
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Signal Transduction
- Molecular Family
- Autophagosome Markers
- Antigen References
-
- Hatanaka H, et al. 2009. J. Biol. Chem. 284:15448-57.
- Sarkari F, et al. 2013. J. Biol. Chem. 288:16975-16985.
- Gene ID
- 6233 View all products for this Gene ID
- UniProt
- View information about RPS27A on UniProt.org
Related FAQs
Other Formats
View All RPS27A Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-RPS27A | A18156E | IHC-P,WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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