- Clone
- W16118A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- AML1, CBFA2, PEBP2aB, RUNX1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
607801 | 25 µg | $101 | ||||
607802 | 100 µg | $253 |
RUNX1 belongs to the runt domain family of transcription factors and regulates target gene expression through forming a heterodimeric DNA-binding complex with CBFB. RUNX1 was first identified as a RUNX1-ETO fusion protein in acute myeloid leukaemia (AML) and is frequently mutated in AML and myelodysplastic syndrome due to chromosomal translocation. RUNX1-deficient mice fail to generate hematopoietic stem cells. RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. Impaired differentiation of megakaryocytes and decreased circulating platelet count was observed in the absence of RUNX1. These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human RUNX1 (amino acids 191-453) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500-1:1000 diluted (0.5 – 1 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
In contrast to BioLegend anti-RUNX1 clone 1C5B16, which shows reactivity towards both human and mouse RUNX1, clone W16118A is specific for human RUNX1 and does not cross-react with mouse RUNX1 (please see supplemental data). Clone W16118A (rat host) does not bind RUNX1 as strongly as 1C5B16 (mouse host), but still performed comparably to a competitor’s antibody.
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). - RRID
-
AB_2721748 (BioLegend Cat. No. 607801)
AB_2721748 (BioLegend Cat. No. 607802)
Antigen Details
- Structure
- 453 amino acids encode a protein with a predicted MW of 48.7 kD. Contains a Runt domain responsible for DNA binding.
- Distribution
-
Nucleus
- Function
- Plays crucial roles in hematopoiesis, hair follicle development, neurogenesis, chondrogenesis, and leukemogenesis.
- Interaction
- Interacts with TLE1, ELF1, ELF2, ELF4, SPI1, KAT6A, KAT6B, YAP1, and CDK6. Forms a heterodimer with CBFB.
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Goyama S, et al. 2013. J. Clin. Invest. 123:3876.
2. Wang J, et al. 2013. PLoS One. 18:e74255.
3. Taniuchi I, et al. 2012. EMBO J. 31:4098.
4. Neel BG, et al. 2012. Genes Dev. 26:1520.
5. Della Gatta G, et al. 2012. Nat. Med. 18:436.
6. Hoi CS, et al. 2010. Mol. Cell Biol. 30:2518. - Gene ID
- 861 View all products for this Gene ID
- UniProt
- View information about RUNX1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All RUNX1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-RUNX1 | W16118A | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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