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Purified anti-RUNX1 Antibody

Pricing & Availability
Clone
W16118A (See other available formats)
Regulatory Status
RUO
Other Names
AML1, CBFA2, PEBP2aB, RUNX1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16118A_PURE_RUNX1_Antibody_WB_1_101217
Total cell lysates (15 µg protein) from SH-SY5Y (lane 1, negative control), U937 (lane 2, negative control), or HEL (lane 3) cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-RUNX1 antibody (Clone W16118A, left) or a competitor’s clone used at the manufacturer’s recommended concentration (right). Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG for clone W16118A, and HRP donkey anti-rabbit IgG for the competitor’s clone, each at a 1:3000 dilution. Membranes were then probed with Direct-Blot™ HRP anti-β-actin antibody at a 1:5000 dilution (lower) to confirm equal protein loading. Lane M: Molecular weight ladder.
  • W16118A_PURE_RUNX1_Antibody_WB_1_101217
    Total cell lysates (15 µg protein) from SH-SY5Y (lane 1, negative control), U937 (lane 2, negative control), or HEL (lane 3) cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-RUNX1 antibody (Clone W16118A, left) or a competitor’s clone used at the manufacturer’s recommended concentration (right). Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG for clone W16118A, and HRP donkey anti-rabbit IgG for the competitor’s clone, each at a 1:3000 dilution. Membranes were then probed with Direct-Blot™ HRP anti-β-actin antibody at a 1:5000 dilution (lower) to confirm equal protein loading. Lane M: Molecular weight ladder.
  • W16118A_PURE_RUNX1_Antibody_WB_2_101217
    Total cell lysates (15 µg protein) from EL4 (lane 1) or HEL (lane 2) cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-RUNX1 antibody (Clone W16118A). Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG. Membranes were then probed with Direct-Blot™ HRP anti-β-actin antibody at a 1:5000 dilution (lower) to confirm equal protein loading. Lane M: Molecular weight ladder.
Cat # Size Price Quantity Check Availability Save
607801 25 µg $101
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607802 100 µg $253
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Description

RUNX1 belongs to the runt domain family of transcription factors and regulates target gene expression through forming a heterodimeric DNA-binding complex with CBFB. RUNX1 was first identified as a RUNX1-ETO fusion protein in acute myeloid leukaemia (AML) and is frequently mutated in AML and myelodysplastic syndrome due to chromosomal translocation. RUNX1-deficient mice fail to generate hematopoietic stem cells. RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. Impaired differentiation of megakaryocytes and decreased circulating platelet count was observed in the absence of RUNX1. These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human RUNX1 (amino acids 191-453) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500-1:1000 diluted (0.5 – 1 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

In contrast to BioLegend anti-RUNX1 clone 1C5B16, which shows reactivity towards both human and mouse RUNX1, clone W16118A is specific for human RUNX1 and does not cross-react with mouse RUNX1 (please see supplemental data). Clone W16118A (rat host) does not bind RUNX1 as strongly as 1C5B16 (mouse host), but still performed comparably to a competitor’s antibody.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2721748 (BioLegend Cat. No. 607801)
AB_2721748 (BioLegend Cat. No. 607802)

Antigen Details

Structure
453 amino acids encode a protein with a predicted MW of 48.7 kD. Contains a Runt domain responsible for DNA binding.
Distribution

Nucleus

Function
Plays crucial roles in hematopoiesis, hair follicle development, neurogenesis, chondrogenesis, and leukemogenesis.
Interaction
Interacts with TLE1, ELF1, ELF2, ELF4, SPI1, KAT6A, KAT6B, YAP1, and CDK6. Forms a heterodimer with CBFB.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Goyama S, et al. 2013. J. Clin. Invest. 123:3876.
2. Wang J, et al. 2013. PLoS One. 18:e74255.
3. Taniuchi I, et al. 2012. EMBO J. 31:4098.
4. Neel BG, et al. 2012. Genes Dev. 26:1520.
5. Della Gatta G, et al. 2012. Nat. Med. 18:436.
6. Hoi CS, et al. 2010. Mol. Cell Biol. 30:2518.

Gene ID
861 View all products for this Gene ID
UniProt
View information about RUNX1 on UniProt.org
Go To Top Version: 0    Revision Date: 10/13/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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