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Purified anti-RXRα Antibody

Pricing & Availability
Clone
W20284A (See other available formats)
Regulatory Status
RUO
Other Names
NR2B1, RXR alpha, RXR-alpha, Retinoid X receptor alpha, Nuclear receptor subfamily
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A_W20284A_PURE_RXRalpha_Antibody_WB_081622
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane, and probed with 1 µg/mL of purified anti-RXRα (clone W20284A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection reagent. Lane M: Molecular weight marker
  • A_W20284A_PURE_RXRalpha_Antibody_WB_081622
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane, and probed with 1 µg/mL of purified anti-RXRα (clone W20284A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection reagent. Lane M: Molecular weight marker
  • B_W20284A_PURE_RXRalpha_Antibody_ICC_110422
    ICC staining of purified anti-RXRα (clone W20284A) on HaCaT cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100, and blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 5.0 μg/mL of the primary antibody, followed by incubation with 2.5 μg/mL of Alexa Fluor® 488 goat anti-rat IgG (Cat. No. 405418) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801), and the image was captured with a 40X objective, Scale bar = 25μm.
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606951 25 µg $110
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606952 100 µg $275
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Description

The human RXR (retinoid X receptor) includes three distinct genes (RXRα, β, and γ). RXR is composed of two major domains of nuclear receptor family proteins, the DNA-binding domain (DBD) and the ligand binding domain (LBD) that bind with high affinity to 9-cis –RA (Retinoic Acid). The RAR/RXR heterodimers bind to RA response elements (RARE) composed of two 5'-AGGTCA-3' sites to modulate RA-mediated gene expression. 

The RXRα, also known as NR2B1, plays a crucial role in various biological effects, such as UVR-induced DNA damage, cell growth, development, metabolism, differentiation, and apoptosis. RXRα is highly expressed in liver and also expressed in kidney and brain. It regulates many intracellular receptor signaling pathways via interacting with ligands and co-regulators. The RXRα/PPARα heterodimer is essential for energy balance regulation such as PPARα transcriptional activity on fatty acid oxidation genes including ACOX1 and the P450 system genes. Proteolytic cleavage of RXRα (tRXRα) protein results in a truncated product found in many tumor cells. The tRXRα (RXRα-Δ80) interacts with the p85α subunit of the PI3K/AKT pathway and leads to the increase of tumor cell proliferation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial Recombinant human RXRα protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The clone does not cross-react with mouse and rat RXRα. 

For ICC, we recommend 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice-cold methanol. We do not recommend ice-cold methanol for fixation and permeabilization.

The clone is not suitable for IHC.

RRID
AB_2924620 (BioLegend Cat. No. 606951)
AB_2924620 (BioLegend Cat. No. 606952)

Antigen Details

Structure
RXRα is a 462 amino acid protein with a predicted molecular weight of 51 kD
Distribution

Expressed in lung fibroblasts, monocytes, kidney and brain

Function
Transcription factor, cell growth and apoptosis
Interaction
RARA, NCOA3, NCOA6, PELP1, SENP6, SFPQ, DNTTIP2, RNF8, BHLHE40/DEC1, BHLHE41/DEC2, NCOR1 and NCOR2
Ligand/Receptor
9-cis retinoic acid
Cell Type
Fibroblasts, Monocytes
Biology Area
Transcription Factors
Molecular Family
Steroid Receptors/Nuclear Receptors
Antigen References
  1. Dawson MI, et al. 2012. Biochim Biophys Acta. 1821:21-56.
  2. Rastinejad F, et al. 2000. EMBO J. 19:1045-54.
  3. Romert A, et al. 1998. Proc Natl Acad Sci U S A. 95:4404–9.
  4. Gorla-Bajszczak A, et al. 1999. Mol Cell Endocrinol. 147:37-47.
  5. Wang Z, et al. 2011. J Invest Dermatol. 131:177-87.
  6. Zhang R, et al. 2018. Cell Death Dis. 9:532.
  7. Wendling O, et al. 1999. Proc Natl Acad Sci U S A. 96:547-51.
  8. Chen L, et al. 2018. Cell Mol Biol Lett. 23:36.
Gene ID
6256 View all products for this Gene ID
UniProt
View information about RXRalpha on UniProt.org
Go To Top Version: 2    Revision Date: 11/04/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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