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Purified anti-S100A8 Antibody

Pricing & Availability
Clone
A22124D (See other available formats)
Regulatory Status
RUO
Other Names
S100 Calcium-binding protein A8, Cystic Fibrosis Antigen (CFAG), Calgranulin A (CAGA, CGLA), Myeloid-related protein 8 (MRP8), S10A8
Isotype
Mouse IgG2b, λ
Ave. Rating
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Product Citations
publications
A22124D_Purified_anti-S100A8_Antibody_1_101424
Whole cell extracts (15 μg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-S100A8 (clone A22124D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A22124D_Purified_anti-S100A8_Antibody_1_101424
    Whole cell extracts (15 μg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-S100A8 (clone A22124D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A22124D_Purified_anti-S100A8_Antibody_2_101424
    IHC staining with Purified anti-S100A8 (clone A22124D) on formalin-fixed paraffin-embedded human tonsil. Following antigen retrieval using 1X Citrate Buffer, 10X (Cat. No. 420901), the tissue was incubated without (panel A) or with (panel B) Purified anti-S100A8 (clone A22124D) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue). Images were captured with a 40X objective. Scale: 50 µm
  • A22124D_Purified_anti-S100A8_Antibody_3_101424
    Human PBMCs were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified Mouse IgG2b, κ Isotype Control (panel A) or Purified anti-S100A8 (clone A22124D) (panel B), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) (red) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged (panels C and D). Scale bar: 50 μm
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673451 25 µg $155
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673452 100 µg $430
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Description

S100A8 is a member of the S100/calgranulin family of proteins containing 2EF-hand calcium-binding motifs. S100A8 is mainly expressed in cells of the myeloid lineage and is present in circulating neutrophils and monocytes. S100A8 expression is also transiently induced in keratinocytes after epidermal injury and in psoriasis where pro-inflammatory cytokines induce its expression. S100A8 is mostly cytoplasmic, but can be translocated to plasma membrane or released into the extracellular environment upon cellular activation. S100A8 preferentially interacts with S100A9 to form a heterodimer S100A8/A9, also known as calprotectin. S100A8/A9 functions as a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation end products (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. The extracellular functions of S100A8/A9 also include the recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. S100A8/A9 displays apoptosis-inducing activity against various tumor cells and also has antimicrobial activity by depriving bacterial pathogens of essential trace metals such as Zn2+ and Mn2+.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human S100A8
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:

  • Fixation buffer (Cat. No. 420801) followed by 100% ice-cold methanol
  • 100% ice-cold methanol only

Antigen Details

Structure
Homodimer
Distribution

Neutrophils, Monocytes, Keratinocytes

Function
Pro-inflammatory molecule
Interaction
Neutrophils, Monocytes
Ligand/Receptor
Toll-like receptor 4 (TLR4) and receptor for advanced glycation end products (AGER)
Biology Area
Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Ubiquitin/Protein Degradation
Molecular Family
Heat Shock Proteins
Antigen References
  1. Bhardwaj RS, et al. 1992 Eur. J. Immunol. 22:1891.
  2. Hunter MJ, et al. 1998 J. Biol. Chem. 273:12427.
  3. Hofmann MA, et al. 1999 Cell 97:889-901.
  4. Cross SS, et al. 2005 Histopathology 46:256.
  5. Zenz R, et al. 2005 Nature 437:369.
  6. Hermani A, et al. 2006 Exp. Cell Res. 312:184.
  7. Vogl T, et al. 2007 Nature Med. 13:1042.
  8. Benedyk M, et al. 2007 J. Invest. Dermatol. 127:2001.
  9. Corbin BD, et al. 2008 Science 319:962.
  10. Gebhardt C, et al. 2008 J. Exp. Med. 205:275.
  11. Loser K, et al. 2010 Nat Med. 16:713-17.
  12. Kang JH, et al. 2015 Immunology 144:79-90.

 

Gene ID
6279 View all products for this Gene ID
UniProt
View information about S100A8 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/14/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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