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Purified anti-SAMHD1 Antibody

Pricing & Availability
Clone
W19081C (See other available formats)
Regulatory Status
RUO
Other Names
SAM and HD Domain Containing Deoxynucleoside Triphosphate, Triphosphohydrolase 1, Monocyte Protein 5, Aicardi-Goutieres Syndrome 5, Sterile Alpha Motif and Histidine Aspartate Domain-Containing Protein 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19081C_PURE_SAMHD1_Antibody_1_110220.png
Whole cell extracts (15 µg total protein) from the indicated cells and tissue were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-SAMHD1 antibody (clone W19081C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19081C_PURE_SAMHD1_Antibody_1_110220.png
    Whole cell extracts (15 µg total protein) from the indicated cells and tissue were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-SAMHD1 antibody (clone W19081C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19081C_PURE_SAMHD1_Antibody_2_110220.png
    Whole cell extracts (250 µg total protein) prepared from Daudi cells were immunoprecipitated overnight with 2.5 µg purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400501) or purified anti-SAMHD1 antibody (clone W19081C). The resulting IP fractions and whole cell extract input (10%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with clone W19081C. Lane M: Molecular weight marker.
  • W19081C_PURE_SAMHD1_Antibody_3_110124
    Human peripheral blood mononuclear cells were fixed and permeabilized with True-Phos™ Perm Buffer set (Cat. No. 425401), and intracellularly stained with purified anti-SAMHD1 (clone W19081C) (left) or purified rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE. Cells were then stained with anti-human CD3 (UCHT1) Pacific Blue™
  • W19081C_PURE_SAMHD1_Antibody_4_110124
    THP1 cells were fixed and permeabilized using True-Phos™ Perm Buffer set (Cat. No. 425401), and intracellularly stained with purified anti-SAMHD1 (clone W19081C) (closed histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
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941001 25 µg $118
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941002 100 µg $293
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Description

SAMHD1 is a host defense factor involved in the innate immune response to viral infections, including HIV-1. Through its dNTPase activity, SAMHD1 disrupts viral genome replication by depleting available pools of deoxynucleotide triphosphates (dNTPs) for viral reverse transcription in dendritic and myeloid cells. In addition to its role in innate immune defense, SAMHD1 also plays an essential role in preserving genome stability through maintaining balanced dNTP pools. Independently of its dNTPase activity, SAMHD1 is required for resection of stalled, gapped, or reversed DNA replication forks by stimulating the exonuclease activity of MRE11. SAMHD1-deficient cells show increased dNTPs and genome instability, and an increased accumulation of G1-positive cells. These cells also accumulate single-strand DNA fragments in the cytosol, leading to the induction of pro-inflammatory type 1 interferons through the cGAS-STING pathway. Mutations in the SAMHD1 are found in many cancers, as well as the severe congenital inflammatory disease Aicardi-Goutieres syndrome.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human SAMHD1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For flow cytometric staining using our True-Phos™ Perm Buffer Protocol, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

During PD testing, we were not able to confirm mouse reactivity of W19081C by western blot.

When tested for western blot, W19081C detected a ~30 kD and 60 kD protein of unknown origin in THP-1 lysates.

Clone W19081C failed immunocytochemistry testing due to non-specific staining. We therefore do not recommend the clone for this application.

RRID
AB_2888902 (BioLegend Cat. No. 941001)
AB_2888902 (BioLegend Cat. No. 941002)

Antigen Details

Structure
SAMHD1 is a 626 amino acid protein with a predicted molecular weight of 72 kD.
Distribution

Ubiquitously expressed/Nucleus

Function
Innate immune response, DNA replication and repair
Cell Type
Dendritic cells, Monocytes
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Innate Immunity
Molecular Family
Enzymes and Regulators
Antigen References
  1. Laguette N, et al. 2011. Nature. 474:654.
Gene ID
25939 View all products for this Gene ID
UniProt
View information about SAMHD1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 11/01/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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