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- Clone
- N127/31 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Disks large-associated protein 1, DAP-1, rGKAP, SAPAP1, PSD-95/SAP90-binding protein 1, SAP90/PSD-95-associated protein 1, guanylate kinase associated protein, guanylate kinase-associated protein, Gkap
- Previously
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Covance Catalog# MMS-5176
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Western blot of purified anti-SAPAP (pan reactive) antibody (clone N127/31). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 832601 | 100 µL | $165 | ||||
SAPAP is also known as DAP-1 or GKAP, encoded by the DLGAP1 gene. It is enriched in the postsynaptic membrane and is responsible for connecting PSD-95 and SHANK, which serves as an adapter protein that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 772-992 (C-terminus) of rat SAPAP1.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB).
- Product Citations
-
- RRID
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AB_2564958 (BioLegend Cat. No. 832601)
Antigen Details
- Structure
- Expected MW: 120 kD (SAPAP2), 110/105/108 kD (SAPAP1/3/4)
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Gene ID
- 9229 View all products for this Gene ID
- UniProt
- View information about SAPAP pan reactive on UniProt.org
Other Formats
View All SAPAP (pan reactive) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-SAPAP (pan reactive) | N127/31 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-SAPAP (pan reactive)
Western blot of purified anti-SAPAP (pan reactive) antibody ...
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