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Purified anti-SARM1 Antibody

Pricing & Availability
Clone
W16079A (See other available formats)
Regulatory Status
RUO
Other Names
Sterile alpha and TIR motif-containing protein 1, Sterile alpha and Armadillo repeat protein, Sterile alpha motif domain-containing protein 2 (SAMD2), MyD88-5, Tir-1 homolog.
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
W16079A_PURE_SARM1_Antibody_042017
Total protein (15µg protein) from EL-4 (negative control), SH-SY5Y, mouse brain and HaCaT (negative control) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 2µg/mL purified anti-SARM1 antibody (clone W16079A ) antibody (upper). Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower).
  • W16079A_PURE_SARM1_Antibody_042017
    Total protein (15µg protein) from EL-4 (negative control), SH-SY5Y, mouse brain and HaCaT (negative control) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 2µg/mL purified anti-SARM1 antibody (clone W16079A ) antibody (upper). Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower).
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696602 100 µg $276
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Description

SARM1 (Sterile alpha and TIR motif-containing protein 1), also known as SAMD2, is a member of the TIR-containing adaptor family. Unlike other TLR adaptor proteins, SARM1 does not activate NF-κB signaling pathway and has been found to be a negative regulator of Toll-Like Receptor-activated transcription. SARM1 is highly expressed in the brain by neurons and has been liked to axonal degeneration following viral infection, axon injury, or oxygen glucose deprivation. Multimerization of SARM1 mediated by the evolutionarily conserved SAM domain is required for its pro-degenerative function. SARM1 also participates in the regulation of neuronal polarity, axonal outgrowth, and brain morphogenesis. Mice deficient in SARM1 demonstrated reduced dendritic arborization and brain size.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial mouse SARM1 recombinant protein (1-410 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2687069 (BioLegend Cat. No. 696602)

Antigen Details

Structure
724 amino acids with a predicted molecular weight of 79.3 kD. Contains two SAM (sterile alpha motif) domains and a TIR (Toll/interleukin-1 receptor) domain.
Distribution

Cytoplasm. Motochondrion.

Function
SARM1 is an adaptor molecule belonging to the TIR-containing adaptor family. SARM1 is a negative regulator of Toll-Like Receptor-mediated signaling and is required for axonal degeneration and brain morphogenesis.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Summers DW, et al. 2016. Proc. Natl. Acad. Sci. USA. 113:E6271.
2. Loreto A, et al. 2015. Cell. Rep. 13:2539.
3. Mukherjee P, et al. 2015. J. Immunol. 195:4913.
4. Gerdts J, et al. 2015. Science. 348:453.
5. Gilley J, et al. 2015. Cell. Rep. 10:1974.
6. Gerdts J, et al. 2013. J. Neurosci. 33:13569.

Gene ID
23098 View all products for this Gene ID
UniProt
View information about SARM1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 04/21/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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