- Clone
- AM009105 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Spike glycoprotein, S glycoprotein (E2)
- Isotype
- Human IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_AM009105_PURE_SARS-CoV-2-S-Protein-S1_Recombinant_Antibody_2_063020.png 1_AM009105_PURE_SARS-CoV-2-S-Protein-S1_Recombinant_Antibody_2_063020.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/AM009105_PURE_SARS-CoV-2-S-Protein-S1_Recombinant_Antibody_2_063020.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Recombinant human ACE2 (Cat. No. 792002) (black squares) binds to immobilized recombinant SARS-CoV-2 S protein S1-Fc chimera (Cat. No. 793004) in a dose-dependent manner. Purified anti-SARS-CoV-2 S protein S1 recombinant antibody (clone AM009105) (purple circles) inhibits the binding in a dose-dependent manner. This antibody blocks the binding of 0.5 µg/mL recombinant human ACE2 to 1.0 µg/mL immobilized recombinant SARS-CoV-2 S protein S1-Fc chimera with an ND50 range of 0.1 - 1.0 µg/mL. -
Recombinant SARS-CoV-2 S protein S1 (Cat. No. 792904) coated onto Nunc™ Maxisorp™ ELISA plates (Cat. No. 423501) at 2 µg/mL and then incubated with a dilution series of purified anti-SARS-CoV-2 S protein S1 recombinant antibody (clone AM009105). Bound antibodies were detected with biotinylated anti-human IgG secondary antibody followed by avidin-horseradish peroxidase and TMB substrate solution. Absorbance was measured at 450 nm.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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938501 | 25 µg | $171 | ||||
938502 | 100 µg | $581 |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single- stranded RNA virus that belongs in a family of viruses known as coronaviruses. SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE-2) expressed on host cells. This protein is composed of two subunits S1 and S2. S1 subunit is responsible for binding to the host cell via ACE-2 receptor and S2 subunit responsible for fusion with the host cell.
This antibody is specific for the S1 subunit of SARS-CoV-2 and was derived, sequenced and expressed from patients that had recovered from COVID-19 infection.
Product Details
- Verified Reactivity
- SARS-CoV-2
- Antibody Type
- Recombinant
- Host Species
- Human
- Immunogen
- SARS-CoV-2
- Formulation
- 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
Block - Quality tested
ELISA - Verified - Recommended Usage
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Each lot is quality control tested by its ability to block the binding between recombinant human ACE2 (Cat. No. 792002) and SARS-CoV-2 S protein S1-Fc chimera (Cat. No. 793004). ND50 range is 0.1 - 1.0 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Recommended concentration for direct ELISA application is 45 ng/mL. - Application Notes
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Note: Clone AM009105 is also referred to as Clone 105-9.
- Additional Product Notes
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Prior to use, quick spin the antibody vial to ensure recovery of maximum volume.
Antibody can be stored in single use aliquots in -20°C for 2 years. Avoid repeated freeze and thaws. -
Application References
(PubMed link indicates BioLegend citation) -
- Wan J, et al. 2020. bioRxiv. doi: https://doi.org/10.1101/2020.05.19.104117
- Product Citations
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- RRID
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AB_2876762 (BioLegend Cat. No. 938501)
AB_2876762 (BioLegend Cat. No. 938502)
Antigen Details
- Structure
- Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 a.a. with a molecular weight of approximately 141 kD and consists of S1 and S2. S1 subunit is 673 a.a. long.
- Distribution
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Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane
- Function
- Host cell surface receptor binding
- Interaction
- ACE2
- Ligand/Receptor
- ACE2
- Biology Area
- COVID-19
- Antigen References
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- Walls AC, et al. 2020. Cell. 181(2):281-292.
- Yan R, et al. 2020. Science. 367 (6485):1444-1448.
- Wrapp D, et al. 2020. Science. 367 (6483):1260-1263.
- Shang J, et al. 2020. PNAS. 117(21):11727-11734
- Gene ID
- 43740568 View all products for this Gene ID
- UniProt
- View information about Viral Protein on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Viral Protein Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-SARS-CoV-2 S Protein S1 Recombinant Antibody | AM009105 | Block,ELISA |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-SARS-CoV-2 S Protein S1 Recombinant Antibody
Recombinant SARS-CoV-2 S protein S1 (Cat. No. 792904) coated... Recombinant human ACE2 (Cat. No. 792002) (black squares) bin...
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