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Purified anti-SARS-CoV S Protein S2 Antibody

Pricing & Availability
Clone
1A9/S2 (See other available formats)
Regulatory Status
RUO
Other Names
Spike glycoprotein, S glycoprotein (E2)
Isotype
Mouse IgG1, κ
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Product Citations
publications
1A9-S2_PURE_SARS-CoV-S-Protein-S2_Antibody_1_120820
Whole cell extracts (15 µg total protein) from 293E cells untransfected (-) or transfected with a plasmid encoding SARS-CoV-2 S protein S2 (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.0125 µg/mL (1:40000 dilution) purified anti-SARS-CoV-2 S protein S2 antibody (clone 1A9/S2) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution and Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • 1A9-S2_PURE_SARS-CoV-S-Protein-S2_Antibody_1_120820
    Whole cell extracts (15 µg total protein) from 293E cells untransfected (-) or transfected with a plasmid encoding SARS-CoV-2 S protein S2 (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.0125 µg/mL (1:40000 dilution) purified anti-SARS-CoV-2 S protein S2 antibody (clone 1A9/S2) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution and Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • 1A9-S2_PURE_SARS-CoV-S-Protein-S2_Antibody_2_120820
    200 ng of recombinant SARS-CoV-2 S Protein S1 + S2 (Cat. No. 793704) was coated onto a Costar™ 96-well high binding assay plate and incubated with a dilution series of purified anti-SARS-CoV-2 S protein S2 antibody (clone 1A9/S2). Bound antibodies were detected with HRP goat anti-mouse IgG antibody (Cat. No. 405306) followed by TMB substrate solution. Absorbance was measured at 450 nm.
  • 1A9-S2_PURE_SARS-CoV-S-Protein-S2_Antibody_3_120820
    200 ng of recombinant SARS-CoV-2 S protein S1 + S2 (green triangles) (Cat. No. 793704), SARS-CoV Protein S1 + S2 (blue circles), and MERS-CoV Protein S1 + S2 (red squares) were coated onto a Costar™ 96-well high binding assay plate and incubated with a dilution series of purified anti-SARS-CoV-2 S protein S2 antibody (clone 1A9/S2). Bound antibodies were detected with HRP goat anti-mouse IgG antibody (Cat. No. 405306) followed by TMB substrate solution. Absorbance was measured at 450 nm.
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944301 25 µg $171
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944302 100 µg $405
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Description

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single-stranded RNA virus that belongs in a family of viruses known as coronaviruses. SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE2) expressed on host cells. This protein is composed of two subunits, S1 and S2. S1 is responsible for binding to the host cell via ACE2 receptor and the S2 subunit is responsible for fusion with the host cell.

Product Details
Technical data sheet

Product Details

Verified Reactivity
SARS-CoV-2
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant SARS-CoV protein corresponding to region of S2 subunit
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ELISA - Verified
Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.0125 - 0.1µg/mL. For ELISA applications, a concentration of 30.4 ng/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

BioLegend offers two clones, 1A9/S2 and A20085C, that recognize SARS-CoV-2 S protein S2.

Both clones have been verified for western blot and direct ELISA, but clone 1A9/S2 is considerably more sensitive for both applications.

Clone 1A9/S2 has been reported to show reactivity towards SARS-CoV (Ng OW, et al. 2014. PLoS One. 9:e102415; Lip KM, et al. 2006. J Virol. 80:941-950.)

Application References

(PubMed link indicates BioLegend citation)
  1. Ng OW, et al. 2014. PLoS One. 9:e102415. (WB, Block)
  2. Lip KM, et al. 2006. J Virol. 80:941-950. (Block)
RRID
AB_2890871 (BioLegend Cat. No. 944301)
AB_2890871 (BioLegend Cat. No. 944302)

Antigen Details

Structure
Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 amino acids with a theoretical molecular weight of 141 kD, and consists of S1 and S2 subunits. The S2 domain is 587 amino acids long with a predicted molecular weight of 64 kD.
Distribution

Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane

Function
Mediates fusion between virus and host cell membranes
Interaction
ACE2
Ligand/Receptor
ACE2
Biology Area
COVID-19
Antigen References
  1. Walls AC, et al. 2020. Cell. 181:281-292.
  2. Yan R, et al. 2020. Science. 367:1444-1448.
  3. Wrapp D, et al. 2020. Science. 367:1260-1263.
  4. Shang J, et al. 2020. Proc Natl Acad Sci U S A. 117:11727-11734.
Gene ID
43740568 View all products for this Gene ID
UniProt
View information about Viral Protein on UniProt.org
Go To Top Version: 1    Revision Date: 12/08/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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