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Purified anti-SEC13 Antibody

Pricing & Availability
Clone
W18034A (See other available formats)
Regulatory Status
RUO
Other Names
SEC13-like protein 1, SEC13-related protein; nuclear pore and COPII coat complex component
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18034A_PURE_SEC13_Antibody_081519.png
Whole cell extracts (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a 1.0 µg/mL (1:500 dilution) of purified anti-SEC13 antibody, clone W18034A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP-conjugated goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W18034A_PURE_SEC13_Antibody_081519.png
    Whole cell extracts (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a 1.0 µg/mL (1:500 dilution) of purified anti-SEC13 antibody, clone W18034A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP-conjugated goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
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671501 25 µg $101
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671502 100 µg $253
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Description

SEC13 functions as a component of the nuclear pore complex (NPC) involved in nucleo-cytoplasmic trafficking. At the ER, SEC13 is involved in the biogenesis of COPII-coated vesicles, which are required for protein export from the ER to either the Golgi complex or the ER-Golgi intermediate compartment (ERGIC). It also functions as a component of the GATOR2 complex, which acts as an amino acid-sensing branch of the TOR1 signaling pathway.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial-length human SEC13 recombinant protein at 123-322 a.a.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone failed IP testing when the target was pulled down and probed via WB.

In addition, a nonspecific band was detected in the initial Western Blot screen; however, that band was titrated out at ~0.1 µg/mL. Nevertheless, the presence of the band indicates that the clone is not suitable for ICC testing.

RRID
AB_2814534 (BioLegend Cat. No. 671501)
AB_2814534 (BioLegend Cat. No. 671502)

Antigen Details

Structure
SEC13 is a 322 amino-acid protein with a predicted molecular weight of 39 kD.
Distribution

Localized to the nucleoplasm and vesicles; ubiquitously expressed.

Function
mTOR signaling; vesicle biogenesis
Biology Area
Protein Trafficking and Clearance
Antigen References
  1. Swaroop A, et al. 1994. Hum. Mol. Genet. 3(8):1281-6.
  2. Wolfson RL, et al. 2017. Nature 543(7645): 438-442.
  3. Cho HJ, et al. 2014. EMBO J 33(20):2314-31.
  4. Chantranupong L, et al. 2014. Cell Rep. 9(1):1-8.
  5. Bar-Peled L, et al. 2013. Science. 340(6136):1100-6.
Gene ID
6396 View all products for this Gene ID
UniProt
View information about SEC13 on UniProt.org
Go To Top Version: 1    Revision Date: 08/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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