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Purified anti-SENP2 Antibody

Pricing & Availability
Clone
W17126B (See other available formats)
Regulatory Status
RUO
Other Names
SUMO Specific Peptidase 2, SUMO1/Sentrin/SMT3 Specific Peptidase 2, SUMO1/Sentrin/SMT3 Specific Protease 2, Sentrin/SUMO-Specific Protease SENP2, Sentrin (SUMO1) Specific Peptidase 2, SMT3-Specific Isopeptidase 2, Sentrin-Specific Protease 2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17126B_Purified_SENP2_Antibody_052019.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-SENP2 Antibody, clone W17126B, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • W17126B_Purified_SENP2_Antibody_052019.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-SENP2 Antibody, clone W17126B, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Check Availability Save
618401 25 µg $101
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618402 100 µg $253
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Description

In eukaryotes, sumoylation (SUMO) is a post-translational modification that contributes to the degradation of proteins harboring the modification. Sentrin-specific protease 2 (SENP2) is an enzyme that is critical for maintaining SUMO homeostasis. There are six described SENP isoforms; each share a highly- conserved catalytic domain in the C-terminus region. SENP2 is localized to the nucleus and catalyzes the deconjugation of SUMO1/2/3 moieties from target proteins. SENP2 has been shown to regulate the level of p53 by desumoylating Mdm2.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Synthetic human SENP2 peptide corresponding to amino acids 562-578
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

We do not recommended this clone for IP or ICC applications.

RRID
AB_2810677 (BioLegend Cat. No. 618401)
AB_2810677 (BioLegend Cat. No. 618402)

Antigen Details

Structure
SENP2 is a 589 amino acid protein with a predicted molecular weight of 67.9 kD.
Distribution

Nucleus and nuclear pore complex/ubiquitously expressed

Function
Protein turnover
Biology Area
Cell Biology
Molecular Family
Protein Modifiers
Antigen References
  1. Hang J and Dasso M. 2002. J.Biol. Chem. 277:19961
  2. Chiu SY, et al. 2008. PLoS Biol. 6:e310
  3. Nayak A and Müller S. 2014. Genome Biol.15:422
Gene ID
59343 View all products for this Gene ID
UniProt
View information about SENP2 on UniProt.org
Go To Top Version: 1    Revision Date: 05/20/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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