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Purified anti-SHP-1 Antibody

Pricing & Availability
Clone
W17240D (See other available formats)
Regulatory Status
RUO
Other Names
Protein Tyrosine Phosphatase, Non-Receptor Type 6, PTPN6, Hematopoietic Cell Protein-Tyrosine Phosphatase, Protein-Tyrosine Phosphatase SHP-1, Protein-Tyrosine Phosphatase 1C
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17240D_Pure_SHP-1_Antibody_1_061819
Whole cell extracts (15 µg protein) from SH-SY5Y and U87-MG (negative control), MOLT-4, Jurkat, THP-1, and EL4 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of Purified anti-SHP-1 Antibody, clone W17240D, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • W17240D_Pure_SHP-1_Antibody_1_061819
    Whole cell extracts (15 µg protein) from SH-SY5Y and U87-MG (negative control), MOLT-4, Jurkat, THP-1, and EL4 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of Purified anti-SHP-1 Antibody, clone W17240D, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • W17240D_Pure_SHP-1_Antibody_2_061819
    Jurkat cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with ice-cold methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:100 dilution (5.0 µg/mL) of either Purified Rat IgG2b, κ Isotype Control Antibody (Cat. No. 400602, panel A) or Purified anti-SHP-1 Antibody, clone W17240D (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counter-stained with DAPI, and the image was captured with a 60X objective.
  • W17240D_Pure_SHP-1_Antibody_3_061819
    Whole cell extracts (250 µg total protein) prepared from Jurkat cells were immunoprecipitated overnight with 2.5 µg of Purified rat IgG2b, κ Isotype Control Antibody (Cat. No. 400602) or Purified anti-SHP-1 Antibody, clone W17240D. The resulting IP fractions and whole cell extract input (5%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1.0 µg/mL (1:500 dilution) of W17240D. Lane M: Molecular Weight marker.
  • W17240D_Pure_SHP-1_Antibody_4_092523
    K562 cells (negative control, open histogram) or Jurkat cells (positive control, filled histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm buffer set (Cat. No. 426803) and intracellularly stained with purified anti-SHP-1 (clone W17240D), or purified Rat IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 400601), followed by Alexa Fluor™ 647 goat anti-Rat IgG (Cat. No. 405416)
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620301 25 µg $101
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620302 100 µg $253
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Description

SHP-1, also referred to as PTPN6 is an inhibitory protein phosphatase widely expressed in hematopoietic cell lineages that regulates cell cycle, proliferation, and inflammatory pathways. SHP-1 dephosphorylates activating signaling molecules in receptor tyrosine kinase pathways; in T cells it functions as a negative regulator of T cell activation and proliferation. SHP-1 is constitutively bound to the ITIM-containing adaptor molecules such as the inhibitory receptor LAIR-1; activation of SHP-1 results in either direct dephosphorylation of the T cell receptor or downstream effector molecules such as Zap70 and Lck. Reduced expression of SHP1 is found in leukemias, lymphomas, and colorectal cancers due to promoter hypermethylation of the PTPN6 gene.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant protein corresponding to amino acid residues 1-213 of human SHP-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per mL. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

W17240D was tested for ICC using PFA-fixed Jurkat cells permeabilized with either Triton X-100 or methanol. While both permeabilization methods facilitated SHP-1 staining, methanol resulted in considerably stronger staining.

Product Citations
  1. Tathe P, et al. 2022. EMBO J. 41:e109720. PubMed
  2. Lam W, et al. 2023. Front Pharmacol. 13:1095186. PubMed
  3. Huang CY, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID
AB_2810679 (BioLegend Cat. No. 620301)
AB_2810679 (BioLegend Cat. No. 620302)

Antigen Details

Structure
SHP-1 is a 595 amino acid protein with a predicted molecular weight of 68 kD.
Distribution

Cytoplasm and nucleus/hematopoietic cells

Cell Type
T cells, Thymocytes
Biology Area
Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Paulson RF, et al. 1996. Nat Genet. 13:309.
  2. Khoury JD, et al. 2004. Blood. 104:1580.
  3. Paster W, et al. 2015. EMBO J. 34:393.
Gene ID
5777 View all products for this Gene ID
UniProt
View information about SHP-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/25/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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