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Purified anti-SIRT7 Antibody

Pricing & Availability
Clone
W17121A (See other available formats)
Regulatory Status
RUO
Other Names
NAD-dependent protein deacetylase sirtuin-7, Regulatory protein SIR2 homolog 7, SIR2-like protein 7, SIRT2L7, Sirtuin7, sirtuin-7, sirtuin 7
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17121A_PURE_SIRT7_Antibody_1_072418
Total cell lysates (15 µg protein) from HeLa, Jurkat, HEK293 and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose membrane, and probed with 1:1000 (0.5 µg/mL) diluted purified anti-SIRT7 antibody (clone W17121A). Proteins were visualized using 1:3000 diluted HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (1:2000 diluted, clone 2F1-1) was used as a loading control. Lane M is the MW ladder.
  • W17121A_PURE_SIRT7_Antibody_1_072418
    Total cell lysates (15 µg protein) from HeLa, Jurkat, HEK293 and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose membrane, and probed with 1:1000 (0.5 µg/mL) diluted purified anti-SIRT7 antibody (clone W17121A). Proteins were visualized using 1:3000 diluted HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (1:2000 diluted, clone 2F1-1) was used as a loading control. Lane M is the MW ladder.
  • W17121A_PURE_SIRT7_Antibody_2_072418
    Total cell lysate (400 µg protein) from HeLa cells were incubated with 2.5 µg rat IgG2a isotype control (lane 1) or anti-SIRT7 antibody (lane 2) at 4°C for 3 hours, followed by incubating with the Protein-G beads overnight. Immunoprecipitates and total protein (10% of input, lane 3) were eluted with SDS loading buffer and resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:500 (1 µg/mL) diluted purified anti-SIRT7 antibody (clone W17121A). Proteins were visualized using 1:3000 diluted HRP goat anti-rat IgG secondary antibody (Cat. No. 405405) and chemiluminescence detection. Lane M is the MW ladder. IgGHC: IgG heavy chain, IgGLC: IgG light chain.
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666101 25 µg $101
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666102 100 µg $253
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Description

Sirtuins are highly conserved NAD+-dependent deacetylases and function in various biological processes, including metabolism, stress response, senescence, genomic stability, and tumorigenesis. SIRT7 is the only sirtuin predominantly localized in the nucleoli and binds rDNA regulatory sequences. In addition to the function in activating transcription of ribosomal genes (rDNA), SIRT7 has been found to deacetylate histone H3K18Ac and maintain the deacetylated stat. SIRT7 plays a role in cell viability by promoting cell cycle progression and proliferation, as well as inhibiting apoptosis. Misregulated expression of SIRT7 has been linked to the progression of various cancers. SIRT7-deficient mice show shortened lifespan and develop aging related pathologies.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human SIRT7 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml (1:250-1:1000 dilution). For immunoprecipitation, the suggested use of this reagent is 2.5 µg per 400 µg total protein. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone can not be used for ICC application.

RRID
AB_2749942 (BioLegend Cat. No. 666101)
AB_2749942 (BioLegend Cat. No. 666102)

Antigen Details

Structure
400 amino acids with a predicted molecular weight of 45 kD. Contains a sirtuin-type deacetylase domain.
Distribution

Nucleolus

Function
SIRT7 is a histone deacetylase involved in the regulation of cell cycle progression, DNA repair, againg, stress resistance, rDNA transcription, chromatin remodeling, lipid metabolism, and tumorigenesis.
Interaction
Interacts with UBTF, MYBBP1A, SMARCA5/SNF2H, BAZ1B/WSTF, ELK4, histone H2A, and histone H2B.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References
  1. Fang J, et al. 2017. Proc. Natl. Acad. Sci. U S A. 114:E8352.
  2. Tang X, et al. 2017. Nat. Commun. 8:318.
  3. Yu J, et al. 2017. Cell. Rep. 18:1229.
  4. Chen S, et al. 2016. Nat. Commun. 7:10734.
  5. Zhang PY, et al. 2016. Nucleic Acids Res. 44:3629.
  6. Yu H, et al. 2014. Clin. Cancer Res. 20:3434.
Gene ID
51547 View all products for this Gene ID
UniProt
View information about SIRT7 on UniProt.org
Go To Top Version: 1    Revision Date: 07/25/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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