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Purified anti-Spectrin β1 Antibody

Pricing & Availability
Clone
N385/21 (See other available formats)
Regulatory Status
RUO
Other Names
SPTB, SPTB1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
N385-21_Purified_Spectrin-B1_Antibody_1_112018
Western blot of purified anti-Spectrin β1 antibody (clone N385/21). Lane 1: Molecular weight marker; Lane 2: 30 µg of human cerebellum lysate; Lane 3: 10 µg of mouse brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N385-21_Purified_Spectrin-B1_Antibody_1_112018
    Western blot of purified anti-Spectrin β1 antibody (clone N385/21). Lane 1: Molecular weight marker; Lane 2: 30 µg of human cerebellum lysate; Lane 3: 10 µg of mouse brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N385-21_Purified_Spectrin-B1_Antibody_2_112018
    Western blot of purified anti-Spectrin β1 antibody (clone N385/21). Lane 1: Molecular weight marker; Lane 2: 10 µg of human cerebellum lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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856901 25 µg $83
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856902 100 µg $165
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Description

Spectrin β1 (SPTB1) is one of the several beta-spectrins that constitute the major components of the cytoskeletal network in erythrocyte plasma membrane. It binds another structural protein, band 4.1, to form a complex that anchors to the cytoplasmic face of the plasma membrane via Ankyrin, which in turn binds to protein band 3. Mutations in spectrin have been linked to hereditary defects in the erythrocyte, such as hereditary elliptocytosis. Recent studies show that caspase- and calpain-mediated cleavage of spectrin leads to the production of a series of fragments collectively called spectrin breakdown products (SBDPs). Multiple studies have shown that SBDPs may serve as a biomarker for traumatic brain injury (TBI)4.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783465 (BioLegend Cat. No. 856901)
AB_2783465 (BioLegend Cat. No. 856902)

Antigen Details

Structure
SPTB1 is a 2137 amino acid protein with an apparent molecular mass of ~ 246 kD.
Distribution

Tissue Distribution: Brain, bone marrow, epididymis, and heart muscle.
Cellular Distribution: Plasma membrane, cytoskeleton, cytosol, and golgi apparatus.

Function
SPTB1 is a major constituent of the cytoskeletal network of the erythrocyte plasma membrane.
Interaction
EPB41 , actin, ankyrin
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Presynaptic proteins, Scaffold Proteins
Antigen References
  1. Zhang R, et al. 2013. Sci China Life Sci. 56(12):1076
  2. Machnicka B, et al. 2014. Biochim Biophys Acta. 1838(2):620
  3. Baines AK. 2009. Biochem Soc Trans. 37(Pt4):796
  4. Kovesdi E. 2010. Acta Neurochir (Wien). 152(1):1
Gene ID
6710 View all products for this Gene ID
UniProt
View information about Spectrin beta1 on UniProt.org
Go To Top Version: 2    Revision Date: 10/29/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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