Purified anti-Tau, 189-195 Antibody anti-Tau

Pricing & Availability

Clone: 39E10 (See other available formats)
Regulatory Status: RUO
Other Names: Microtubule-associated protein tau, PHF-tau, Paired Helical Filament tau
Previously: Covance Catalog# MMS-5017
Isotype: Mouse IgG1, κ
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Product Citations: publications

39E10_PURE_Tau181-195_WB_113018 — Western blot of purified anti-Tau, 189-195 antibody (clone 39E10). Lane 1: Molecular weight marker; Lane 2: 20 µg of normal human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 0.1 µg of recombinant human Tau protein ladder. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.

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814302

25 µL

$112

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814301

100 µL

$275

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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against full-length human tau.
Formulation: Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in immunoblotting (WB).

BioLegend is the Exclusive Provider of this Antibody Product
RRID: AB_2715844 (BioLegend Cat. No. 814302)
AB_2715844 (BioLegend Cat. No. 814301)

Antigen Details

Structure: Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution: Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: Cytoskeleton, nucleus, plasma membrane, and cytosol.
Function: Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction: Tau interacts with: Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, and alpha-synuclein.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: Tau
Antigen References: 1. Meredith JE Jr, et al. 2013. PLoS One. 8(10): e76523. PubMed
2. Goodall CA, et al. 2006. J. Neurol. Neurosurg. Psychiatry 77(1): 89. PubMed
3. Wang Y, Mandelkow E. 2016. Nat. Rev. Neurosci. 17(1):5-21. PubMed
Gene ID: 4137 View all products for this Gene ID
UniProt: View information about Tau 189-195 on UniProt.org

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Pathways

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Other Formats

View All Tau, 189-195 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Tau, 189-195	39E10	WB
Biotin anti-Tau, 189-195	39E10	WB,Direct ELISA
HRP anti-Tau, 189-195	39E10	WB

Certificate of Analysis

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Go To Top Version: 9 Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

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