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Purified anti-Tau Phospho (Thr231) Antibody

Pricing & Availability
Clone
A23009A (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule-associated protein tau, PHF-tau, paired helical filament-tau, neurofibrillary tangle protein, microtubule-associated protein tau, isoform 4, G protein beta1/gamma2 subunit-interacting factor 1, MAPT
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A. A23009A_PURE_Tau_WB-1_082024
Whole brain lysates (15 µg total protein) from 5 days old (P5) or 31 days old (P31) rats were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-Tau Phospho (Thr231) (clone A23009A) or Pan anti-Tau antibody. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426316) was used as a detection agent. Lane M: Molecular weight marker.
  • A. A23009A_PURE_Tau_WB-1_082024
    Whole brain lysates (15 µg total protein) from 5 days old (P5) or 31 days old (P31) rats were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-Tau Phospho (Thr231) (clone A23009A) or Pan anti-Tau antibody. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426316) was used as a detection agent. Lane M: Molecular weight marker.
  • B. A23009A_PURE_Tau_WB-2_082024
    Whole brain lysates (15 µg total protein) from 5 days old (P5) or 31 days old (P31) rats were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, then untreated (LPP -) or treated (LPP +) with Lambda protein phosphatase. The blots were probed with Purified anti-Tau Phospho (Thr231) (clone A23009A). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426316) was used as a detection agent. Lane M: Molecular weight marker
  • c. A23009A_PURE_Tau_IHC-P_082024
    IHC staining with Purified anti-Tau Phospho (Thr231) on formalin-fixed paraffin-embedded human Alzheimer’s Disease cortex tissue (panels A and B) or normal human cortex tissue (panels C and D) following antigen retrieval using Citrate Buffer (pH 6.0) (Cat. No. 420902). The tissue was incubated without (panels A and C) and with (panels B and D) Purified anti-Tau Phospho (Thr231) (clone A23009A) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
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683852 100 µg $455
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Description

Paired helical filaments (PHFs) are the major building blocks of neurofibrillary lesions in Alzheimer's disease brains and are composed of hyperphosphorylated Tau protein. Predominantly expressed in axons, alternatively spliced forms of Tau comprise a family of microtubule-associated proteins that normally promote and stabilize the assembly of microtubules. PHF-Tau differs from normal Tau by its abnormal hyperphosphorylation, which results in decreased binding of Tau to microtubules. The decreased affinity of PHF-Tau for microtubules, coupled with reduced levels of normal Tau, destabilizes microtubules leading to an impairment of axonal transport, neuronal death and the aggregation of PHFs. Therefore, hyperphosphorylation of Tau is believed to be a key event in the pathogenesis of Alzheimer's disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic human Tau peptide phosphorylated at Thr231
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use in immunohistochemistry on FFPE tissues (IHC-P), we recommend Citrate buffer (Cat. No. 420902) for antigen retrieval.

RRID
AB_3662423 (BioLegend Cat. No. 683852)

Antigen Details

Structure
Tau proteins constitute a family of six isoforms with the range from 352-441 amino acids with molecular weights from 55 kD to 78 kD.
Distribution

Microtubules associated in axons of neurons of the central nervous system

Function
Maintain stability of microtubules in neurons. It is regulated by phosphorylation.
Interaction
Tubulin microtubules
Cell Type
Neurons
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Neurodegeneration, Neuroscience Cell Markers, Protein Misfolding and Aggregation
Molecular Family
Intermediate Filaments, Tau
Antigen References
  1. Lasagna-Reeves CA, et al. 2012. FASEB J. 26:1946-1959.
  2. Mitchell TW, et al. 2000. J Histochem Cytochem. 48:1627-1637.
  3. Hong M, et al. 1997. J Biol Chem. 272:25326-25332.
  4. Bramblett GT, et al. 1993. Neuron. 10:1089-1099.
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau Phospho Thr231 on UniProt.org
Go To Top Version: 1    Revision Date: 08/20/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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