- Clone
- P82A8A6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Zinc finger E-box-binding homeobox 1, Negative regulator of IL2, Transcription factor 8, ZEB1, BZP, AREB6
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
685602 | 100 µg | $265 |
TCF-8, also named ZEB1, belongs to the ZEB family of transcription factors characterized by the presence of 2 zinc finger clusters and are responsible for the binding to specific DNA sequences named E-boxes. By recruiting co-suppressors or co-activators through CID, SID or CBD, TCF-8 can either downregulate or upregulate the expression of its target genes.
TCF-8 promotes tumor invasion and metastasis by inducing epithelial-mesenchymal transition (EMT) in carcinoma cells. It is associated with aggressive behaviour, metastasis, treatment resistance and poor prognosis in different tumour types, including breast, pancreatic, prostate and lung cancer.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant fragment of human ZEB1 (aa 967-1108) expressed in E. Coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2572205 (BioLegend Cat. No. 685602)
Antigen Details
- Structure
- Major isoform has 1,124 amino acids and a predicted molecular weight of 120 kD.
- Distribution
-
Nucleus.
- Function
- Transcriptional repressor of IL-2, E-cadherin, BCL6, and RCOR1. Enhances or represses the promoter activity of the ATP1A1 gene, positively regulates neuronal differentiation, and promotes tumorigenicity by repressing stemness-inhibiting microRNAs.
- Interaction
- SMARCA4/BRG1.
- Biology Area
- Cell Biology, Signal Transduction, Transcription Factors
- Antigen References
-
1. Postigo AA, et al. 2003. EMBO J. 22:2453.
2. Peinado H, et al. 2007. Nat. Rev. Cancer 7:415.
3. Karihtala P, et al. 2013. Breast Cancer Res. Treat. 138:81.
4. Bronsert P, et al. 2014. Surgery 156:97.
5. Liu Y, et al. 2014. Nat. Commun. 5:5660.
6. Zhang P, et al. 2015. Cell Cycle. 14:481.
7. Lehmann W, et al. 2016. Nat. Commun. 7:10498. - Gene ID
- 6935 View all products for this Gene ID
- UniProt
- View information about TCF8 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All TCF8 (ZEB1) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-TCF8 (ZEB1) | P82A8A6 | WB |
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Purified anti-TCF8 (ZEB1)
Total cell lysates (15 µg protein) from the indicated cell l...
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