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Purified anti-TCF8 (ZEB1) Antibody

Pricing & Availability
Clone
P82A8A6 (See other available formats)
Regulatory Status
RUO
Other Names
Zinc finger E-box-binding homeobox 1, Negative regulator of IL2, Transcription factor 8, ZEB1, BZP, AREB6
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P82A8A6_PURE_TCF8_Antibody_WB_100120
Total cell lysates (15 µg protein) from the indicated cell lines were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TCF8 (ZEB1) Antibody, clone P82A8A6. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse-IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Lane M: Molecular weight ladder.
  • P82A8A6_PURE_TCF8_Antibody_WB_100120
    Total cell lysates (15 µg protein) from the indicated cell lines were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TCF8 (ZEB1) Antibody, clone P82A8A6. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse-IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Lane M: Molecular weight ladder.
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685602 100 µg $265
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Description

TCF-8, also named ZEB1, belongs to the ZEB family of transcription factors characterized by the presence of 2 zinc finger clusters and are responsible for the binding to specific DNA sequences named E-boxes. By recruiting co-suppressors or co-activators through CID, SID or CBD, TCF-8 can either downregulate or upregulate the expression of its target genes.

TCF-8 promotes tumor invasion and metastasis by inducing epithelial-mesenchymal transition (EMT) in carcinoma cells. It is associated with aggressive behaviour, metastasis, treatment resistance and poor prognosis in different tumour types, including breast, pancreatic, prostate and lung cancer.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant fragment of human ZEB1 (aa 967-1108) expressed in E. Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2572205 (BioLegend Cat. No. 685602)

Antigen Details

Structure
Major isoform has 1,124 amino acids and a predicted molecular weight of 120 kD.
Distribution

Nucleus.

Function
Transcriptional repressor of IL-2, E-cadherin, BCL6, and RCOR1. Enhances or represses the promoter activity of the ATP1A1 gene, positively regulates neuronal differentiation, and promotes tumorigenicity by repressing stemness-inhibiting microRNAs.
Interaction
SMARCA4/BRG1.
Biology Area
Cell Biology, Signal Transduction, Transcription Factors
Antigen References

1. Postigo AA, et al. 2003. EMBO J. 22:2453.
2. Peinado H, et al. 2007. Nat. Rev. Cancer 7:415.
3. Karihtala P, et al. 2013. Breast Cancer Res. Treat. 138:81.
4. Bronsert P, et al. 2014. Surgery 156:97.
5. Liu Y, et al. 2014. Nat. Commun. 5:5660.
6. Zhang P, et al. 2015. Cell Cycle. 14:481.
7. Lehmann W, et al. 2016. Nat. Commun. 7:10498.

Gene ID
6935 View all products for this Gene ID
UniProt
View information about TCF8 on UniProt.org
Go To Top Version: 4    Revision Date: 10/01/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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