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Purified anti-TFIIB Antibody

Pricing & Availability
Clone
IIB1 (See other available formats)
Regulatory Status
RUO
Other Names
Transcription initiation factor IIB, General transcription factor TFIIB, S300-II, GTF2B, TF2B, TFIIB
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A-IIBI_Pureaf_TFIIB_Antibody_1_WB_090616
Total cell lysate from E. coli DH5α (lane 1, 15µg), NIH3T3 cells (lane 2, 15µg) and HeLa cells (lane 3, 15µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-TFIIB antibody (clone IIB1). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
  • A-IIBI_Pureaf_TFIIB_Antibody_1_WB_090616
    Total cell lysate from E. coli DH5α (lane 1, 15µg), NIH3T3 cells (lane 2, 15µg) and HeLa cells (lane 3, 15µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-TFIIB antibody (clone IIB1). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
  • B-IIBI_Pureaf_TFIIB_Antibody_2_WB_071018
    Total cell lysates (15 µg protein) from HeLa (lane 1) and NIH3T3 (lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:1000) of Purified anti-TFIIB Antibody (clone IIB1, upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for anti-TFIIB Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin antibody, clone 2F1-1(lower panel) as a loading control. Lane M: Molecular weight ladder.
  • C-IIBI_Pureaf_TFIIB_Antibody_2_IF_050115
    HeLa cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/ml anti-TFIIB (clone IIB1) in blocking buffer overnight at 4°C and followed by DyLight™ 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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668501 100 µg $323
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Description

TFIIB is one of the ubiquitous transcription factors required for transcription initiation by RNA polymerase II. It plays an important role in pre-initiation complex assembly, which provides a bridge between promoter-bound TFIID and RNA polymerase II. Moreover, TFIIB also directly modulates the catalytic center of RNA polymerase II.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein.
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
ICC - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone IIB1 recognizes epitope 1-51.

Application References

(PubMed link indicates BioLegend citation)
  1. Thompson NE, et al. 1995. J. Biol. Chem. 270:4735. (IP)
RRID
AB_2564439 (BioLegend Cat. No. 668501)

Antigen Details

Structure
316 amino acids with a predicted molecular weight of approximately 35 kD.
Distribution

Nucleus.

Function
The Transcription factor is required for transcription initiation by RNA polymerase II.
Interaction
Transcription factors IID and IIA and RNA polymerase II.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Ha I, et al. 1991. Nature 352:689.
3. Hurwitz, J. 2005. J. Biol. Chem. 280:42477.
4. Deng W, Roberts SG. 2007. Chromosoma. 5:417.
5. Hirata A, et al. 2008. Nature 451:851.

Gene ID
2959 View all products for this Gene ID
UniProt
View information about TFIIB on UniProt.org
Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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