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Purified anti-Th-POK (ZFP-67) Antibody

Pricing & Availability
Clone
W15147A (See other available formats)
Regulatory Status
RUO
Other Names
T-helper-inducing POZ/Krueppel-like factor, Zinc finger and BTB domain-containing protein 7B (ZBTB7B), Krueppel-related zinc finger protein (cKrox), Zinc finger protein 67 homolog (ZFP-67), Thpok
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W15147A_PURE_ThdashPOK_Antibody_1_110818
Total cell lysates (15 µg total protein) from MOLT4 (negative control), HEL, MCF-7, HeLa and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TH-POK Antibody, clone W15147A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • W15147A_PURE_ThdashPOK_Antibody_1_110818
    Total cell lysates (15 µg total protein) from MOLT4 (negative control), HEL, MCF-7, HeLa and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TH-POK Antibody, clone W15147A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • W15147A_PURE_ThdashPOK_Antibody_2_110818
    HeLa cells were fixed with 2% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 2.0 µg/ml purified anti-Th-POK antibody (clone W15147A) for one hour, followed by staining with Alexa Fluor® 594 conjugated anti-rat IgG antibody (red). Actin filaments were labeled with Alexa Fluor® 488 conjugated Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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688302 100 µg $265
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Description

Th-POK is a member of the BTB-POZ domain-containing zinc finger transcription factor family. It regulates gene expression during intrathymic T cell differentiation. A single autosomal recessive mutation of the Th-POK gene results in the absence of mature CD4+ helper T cells, called helper-deficient (HD) phenotype. In Th-POK deficient mice, MHC class II restricted thymocytes are redirected to the CD8+ T cell lineage. On the contrary, forced expression of Th-POK redirects MHC class I restricted thymocytes to the CD4+ T cell lineage. Inactivation of Th-POK in mature CD4+ T cells results in induction of the genes preferentially expressed in CD8+ T cells, such as CD8, granzyme B, and IFN-γ. These findings suggest that Th-POK is essential in promoting commitment of immature T cells to the CD4 lineage and maintaining the characteristics of mature CD4+ T helper cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial mouse Th-POK recombinant protein (197-350 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone recognizes mouse TH-POK.

This clone displayed a similar affinity to BioLegend clone 11H11A14 by western blot. However, clone 11H11A14 does not recognize mouse TH-POK.

RRID
AB_2617025 (BioLegend Cat. No. 688302)

Antigen Details

Structure
539 amino acids with a predicted molecular weight of 58 kD and observed of approximately 70 kD; contains four C2H2-type zinc finger regions that are responsible for DNA binding; contains a BTB-POZ domain which is involved in homodimerization.
Distribution

Nucleus.

Function
Transcription factor that regulates CD4 lineage commitment of immature T-cell precursors; acts as a transcriptional repressor of collagen and fibronectin genes.
Interaction
Interacts with the acetyltransferase p300.
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Kappes DJ. 2010. Immunol. Rev. 238:182.
2. Mariani F, et al. 2013. PLoS One 8:e54488.
3. Zhang M, et al. 2010. J. Immunol. 185:3960.
4. Egawa T, et al. 2008. Nat. Immunol. 9:1131.
5. Wildt KF, et al. 2007. J. Immunol. 179:4405.

Gene ID
51043 View all products for this Gene ID
UniProt
View information about Th-POK on UniProt.org
Go To Top Version: 3    Revision Date: 11/08/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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