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Purified anti-TICAM-1 (TRIF) Antibody

Pricing & Availability
Clone
1H4B01 (See other available formats)
Regulatory Status
RUO
Other Names
TIR domain-containing adapter molecule 1, TRIF, MyD88-3, Proline-Rich, Vinculin And TIR Domain-Containing Protein B (PRVTIRB)
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A_1H4B01_PURE_TICAM1_Antibody_WB_113017
Total lysates (15 µg protein) from HepG2 (lane 1) and HeLa (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-TICAM-1 antibody (clone 1H4B01, upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted HRP goat anti-mouse IgG secondary antibody for the anti-TICAM-1 antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, lower). Lane M: Molecular weight ladder.
  • A_1H4B01_PURE_TICAM1_Antibody_WB_113017
    Total lysates (15 µg protein) from HepG2 (lane 1) and HeLa (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-TICAM-1 antibody (clone 1H4B01, upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted HRP goat anti-mouse IgG secondary antibody for the anti-TICAM-1 antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, lower). Lane M: Molecular weight ladder.
  • B_1H4B01_PURE_TICAM1_Antibody_IF_113017
    Raji cells were stained with purified anti-TICAM-1 (clone 1H4B01) antibodies, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
  • C_1H4B01_PURE_TICAM1_Antibody_IP_113017
    Immunoprecipitation of TICAM1 from Raji cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-TICAM1 antibody (clone 1H4B01, lane 4). Western blot was performed using anti-TICAM1 antibody (clone 1H4B01).
  • D_S5-1_PURE_CD88_Antibody_072722
    IHC staining using purified anti-TICAM-1 (TRIF) (clone 1H4B01) on formalin-fixed paraffin-embedded human small intestine tissue. Following antigen retrieval using 1X Tris-EDTA, Ph 9.0, the tissue was incubated with (B) or without (A) 1.0 μg/mL of antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scalebar = 50 μM
  • E_S5-1_PURE_CD88_Antibody_072722
    IHC staining using purified anti-TICAM-1 (TRIF) (clone 1H4B01) on formalin-fixed paraffin-embedded human cortex tissue. Following antigen retrieval using 1X Tris-EDTA, pH 9.0, the tissue was incubated with (B) or without (A) 10.0 μg/mL of antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scalebar = 50 μM
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657102 100 µg $265
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Description

TICAM-1, also known as TRIF, is an intracellular adaptor protein utilized by TLR3 and TLR4. TLR3 recognizes double stranded viral RNA and induces TICAM-1 mediated type I interferon production. The N-terminal region of TICAM-1 recruits TBK1 and IKKi, which in turn phosphorylates the interferon regulatory transcription factor IRF3. The phosphorylated IRF3 is subsequently dimerized and translocates to the nucleus, resulting in transcriptional activation of the gene encoding IFN-β. Mice lacking in TICAM-1 expression are defective in the TLR3 mediated induction of proinflamatory cytokines, suggesting that TICAM-1 is crucial for TLR3 downstream signaling. TICAM-1 also mediates TLR4-induced signaling in the presence of the adaptor molecule TRAM. The C-terminal region of TICAM-1 recruits RIP1 and induces apoptosis through FADD/caspase cascade.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human TICAM-1 recombinant protein (29-204 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 - 10 μg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Oldenburg R, et al. 2018. Front Immunol. 9:2. PubMed
  2. Bhattacharjee P, et al. 2018. Sci Rep. 13:e0199785. PubMed
  3. Liu Z, et al. 2021. Immunity. 54(2):247-258.e7. PubMed
RRID
AB_2562543 (BioLegend Cat. No. 657102)

Antigen Details

Structure
712 amino acids, predicted molecular weight of 76 kD. Contains a Toll/interleukin-1 receptor (TIR) homology domain responsible for mediating protein-protein interactions.
Distribution

Cytosol, endosome membrane

Function
TICAM-1 acts as an adaptor protein of TLR3 and TLR4 (through TRAM), mediating innate immune responses during viral infection.
Interaction
TICAM-1 interacts with TLR3, TRAM, TBK1, IRF3, IRF7, TRAF3, TRAF6, and IKKi.
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Toll Like Receptors
Antigen References

1. Petnicki-Ocwieja T, et al. 2013. Infect Immun. 81:402.
2. Riad A, et al. 2011. J. Immunol. 186:2561.
3. Choi YJ, et al. 2010. J. Biol. Chem. 285:21382.
4. Gais P, et al. 2010. J. Immunol. 184:5842.
5. Han KJ, et al. 2010. J. Biol. Chem. 285:12543.
6. Takeda K and Akira S. 2005. Int. Immunol. 17:1.

Gene ID
148022 View all products for this Gene ID
UniProt
View information about TICAM-1 on UniProt.org
Go To Top Version: 4    Revision Date: 07/26/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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