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Purified anti-TMEM119 (Extracellular) Antibody

Pricing & Availability
Clone
A16075D (See other available formats)
Regulatory Status
RUO
Other Names
Transmembrane protein 119
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A16075D_Pure_TMEM119_Antibody_1_052118
Human Embryonic Kidney 293 (HEK293) cells transfected with human TMEM119* (filled histogram) or transfected with an irrelevant plasmid (open histogram) were stained with 0.6 µg/mL of TMEM119 (Clone A16075D), followed by anti-mouse IgG PE.
  • A16075D_Pure_TMEM119_Antibody_1_052118
    Human Embryonic Kidney 293 (HEK293) cells transfected with human TMEM119* (filled histogram) or transfected with an irrelevant plasmid (open histogram) were stained with 0.6 µg/mL of TMEM119 (Clone A16075D), followed by anti-mouse IgG PE.
  • A16075D_Pure_TMEM119_Antibody_2_052118
    Western blot of anti-TMEM119 (Extracellular) antibody (clone A16075D). Lane 1: Molecular weight marker; Lane 2: Mock transfected HEK293 cell lysate; Lane 3: Lysate of HEK293 cells transfected with human TMEM119*. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). β-actin was used as the loading control (Cat. No. 643807). Enhanced chemiluminescence was used as the detection system.
  • A16075D_Pure_TMEM119_Antibody_3_052118
    ICC staining of anti-TMEM119 (Extracellular) antibody (clone A16075D) on HEK293 cells transfected with human TMEM119*. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 5 µg/mL of the primary antibody for overnight at 4°C, followed by incubation wit 1 µg/mL of Alexa Fluor® 594 goat anti-Mouse IgG (Cat. No. 405326) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
  • A16075D_PURE_TMEM119_Antibody_4_062221.png
    IHC staining of purified anti-TMEM119 antibody (clone A16075D) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 0.1 M Tris-buffered saline with Tween 20 (Cat. No. 925501), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (yellow) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10x objective. Scale bar: 100 µm
  • A16075D_PURE_TMEM119_Antibody_5_081721
    IHC staining of purified anti-TMEM119 antibody (A16075D) on formalin-fixed paraffin-embedded human neocortical brain tissue. Following antigen retrieval using T.E. pH 9.0, the tissue was incubated with 0.5 µg of primary antibody for 1.5 hours at room temperature, followed by incubation with 1:200 dilution of Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity) antibody (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The image was captured with a 40X objective. Scale bar: 50 µm.
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853301 25 µg $112
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853302 100 µg $288
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Description

Microglia are the resident parenchymal myeloid cells of the CNS, and play an important role in development, homeostasis, disease, and injury. TMEM119 is a transmembrane protein and was identified as a microglia-specific marker in both mouse and human CNS. Moreover, TMEM119 immunoreactivity has been exclusively detected on a subset of Iba1(+) CD68(+) microglia with ramified and amoeboid morphologies in the brains of neurodegenerative diseases. TMEM119 also plays an important role in bone formation and normal bone mineralization. It promotes the differentiation of myoblasts into osteoblasts. and it may induce the commitment and differentiation of myoblasts into osteoblasts. TMEM119 is also essential for normal spermatogenesis and late testicular differentiation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human TMEM119 protein (extracellular domain)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB, ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.6 - 5.0 µg/mL. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per mL. For immunocytochemistry, a concentration range of 2.0 - 5.0 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 µg/ml is suggested.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For microscopy, the antibody was found to work best when using fresh frozen human tissues. Communication by Dr. Frederick Christian Bennett at Stanford University, under review in Neuron.

*The human TMEM119 full length plasmid was a courtesy gift from Dr. Ben Barres at Stanford University.

Application References

(PubMed link indicates BioLegend citation)

  1. Bennett FC, et al. 2018. Neuron. 98:1-14 PubMed

Product Citations
  1. Tang Y, et al. 2023. J Clin Invest. 133:. PubMed
  2. Patel T, et al. 2022. Aging Cell. 21:e13606. PubMed
  3. Bodnar B, et al. 2021. Front Cell Neurosci. 15:682272. PubMed
  4. Kumar A, et al. 2021. EBioMedicine. 63:103192. PubMed
  5. Ruan C, et al. 2020. Brain Behav Immun. 83:180. PubMed
  6. Rai MA, et al. 2020. Retrovirology. 17:35. PubMed
  7. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  8. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  9. Chen P, et al. 2020. Cancer Discov. 0.674305556. PubMed
  10. Toedebusch RG, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_2734647 (BioLegend Cat. No. 853301)
AB_2734647 (BioLegend Cat. No. 853302)

Antigen Details

Structure
TMEM119 is an 283 amino acid protein with a molecular mass of 30 kD.
Distribution

Tissue distribution: TMEM119 is expressed in the brain, bone, and lymphoid tissues.
Cellular distribution: Membrane and endoplasmic reticulum.

Function
TMEM119 is an important microglia marker and is implicated in neurodegenerative diseases.
Interaction
TMEM119 interacts with SMAD1, SMAD5 and RUNX2.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Bennett ML, et al. 2016. Proc Natl Acad Sci U S A. 113
  2. Satoh J, et al. 2016. Neuropathology. 36:39-49
  3. Kawao N and Kaji H. 2015. J Cell Biochem. 116:687
  4. Zrzavy T, et al. 2017. Brain. 140:1900-1913
Gene ID
338773 View all products for this Gene ID
UniProt
View information about TMEM119 on UniProt.org
Go To Top Version: 3    Revision Date: 06/22/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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