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Purified anti-TOMM40 Antibody

Pricing & Availability
Clone
A17123A (See other available formats)
Regulatory Status
RUO
Other Names
Mitochondrial import receptor subunit TOM40 homolog
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A17123A_PURE_TOMM40_Antibody_07202018
Western blot of purified anti-TOMM40 antibody (clone A17123A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.001 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. The band at ~60 kD is potentially the TOM/TIM complex.
  • A17123A_PURE_TOMM40_Antibody_07202018
    Western blot of purified anti-TOMM40 antibody (clone A17123A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.001 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. The band at ~60 kD is potentially the TOM/TIM complex.
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853601 25 µg $106
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853602 100 µg $276
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Description

Mitochondria fulfill central functions in cellular energetics, metabolism, and signaling. The outer membrane translocator complex (the TOM complex) imports most mitochondrial proteins, and TOMM40 is the key subunit of the complex, the pore through which the vast majority of imported proteins must pass. TOMM40 possesses chaperone-like properties, which helps expedite movement of the pre-proteins through the channel. TOM cooperates with the TIM23 (Translocase of the Inner Membrane 23) complexes to import matrix-targeted proteins. TOMM40 is implicated in many neurodegenerative diseases; reduced TOMM40 mRNA expression in Alzheimer’s disease patients was shown to correlate with cognitive decline.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
A synthetic peptide corresponding to TOMM40 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody shows negligible cross-reactivity with human TOMM40.

RRID
AB_2749940 (BioLegend Cat. No. 853601)
AB_2749940 (BioLegend Cat. No. 853602)

Antigen Details

Structure
TOMM40 is a 361 amino acid protein with a molecular weight mass of ~40 kD.
Distribution

Tissue distribution: TOMM40 is expressed in numerous tissues including the central nervous system (CNS), muscle, and the immune systems.
Cellular distribution: Cytosolic, mitochondria, and nucleus.

Function
TOMM40 is the key subunit of the TOM complex, which imports proteins into the mitochondria.
Interaction
TOMM40 Interacts with mitochondrial targeting sequences and the TIM complex.
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Transcription Factors
Molecular Family
Mitochondrial Markers
Antigen References
  1. Gottschalk WK, et al. 2014. J. Parkinsons Dis. Alzheimers Dis. 12.
  2. Shiota T, et al. 2015. Science 349:1544-8.
  3. Mise A, et al. 2017. J. Alzheimers Dis. 60:1107-1117.
  4. Ahting U, et al. 2001. J. Cell. Biol. 153:1151-60.
Gene ID
10452 View all products for this Gene ID
UniProt
View information about TOMM40 on UniProt.org
Go To Top Version: 1    Revision Date: 07/24/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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